改良的蛋白质电泳考马斯亮蓝染色方法研究  

The Research of an Improved Method for Protein Staining in Electrophoresis with Coomassie Brilliant Blue

作　　者：饶维桥 肖伟敏 张继远 饶媛 訾金 

机构地区：[1]深圳华大基因研究院质谱平台,深圳518083

出　　处：《生物技术通报》2014年第9期58-64,共7页Biotechnology Bulletin

基　　金：国家"863"课题资助项目(2012AA020204)

摘　　要：旨在建立简便、经济、灵敏以及质谱兼容的蛋白质聚丙烯酰胺凝胶电泳染色法,在胶体考染(Blue Silver)配方(0.12%的考马斯亮蓝G-250,10%的磷酸,10%的硫酸铵,和20%的甲醇)的基础上,通过优化凝胶固定时间与染色液中各物质用量,得到改良的染色液配方(0.1%的考马斯亮蓝G-250或R-250,5%的磷酸,5%的硫酸铵,10%的甲醇)及简便的操作步骤。试验对比结果显示,改良方法一与胶体考染相比仍保持了较高的染色灵敏度以及较好的质谱兼容性,且有机试剂使用量更少,染色深度与蛋白质量的线性关系也更好。In order to establish a convenient, sensitive, economic and Mass Spectrometry compatible staining method for protein polyacrylamide gel electrophoresis, herein, we established optimized staining methods based on Blue Silver Staining, by optimizing the gel immobilization time and buffer content. Comparison result indicated the method 1 keep the similar high sensitivity and well Mass Spectrometry compatible feature as Blue Silver Staining, what＇ s more, is more easily conducted, less organic solvent consuming and have better correlation between protein amount and staining density.

关 键 词：蛋白质染色 考马斯亮蓝 灵敏度 线性范围 经济环保 

分 类 号：Q503[生物学—生物化学]