高粱EMS诱变及突变体筛选、鉴定  

EMS Mutagenesis,Mutant Screening and Identification of Sorghum

作　　者：王春语[1] 朱振兴[1] 李丹[1] 丛玲[1] 张丽霞[1] 

机构地区：[1]辽宁省农业科学院创新中心,沈阳110161

出　　处：《生物技术通报》2014年第9期78-83,共6页Biotechnology Bulletin

基　　金：农业部现代农业产业技术体系项目(CARS-06-01-06)

摘　　要：突变率和突变类型是突变体表型筛选和构建饱和突变体库的重要依据。采用不同时间和不同浓度甲基磺酸乙酯(EMS)处理高粱品种BTx623的种子,突变率和突变类型差异较大。突变类型可分为叶色、叶形、穗型、育性及生育期等,变异类型较丰富。根据处理后种子出苗率、结实率和突变率的情况,最终确定20 h+EMS 0.2%为最佳的诱变处理时间和浓度。但构建饱和突变体库,建议采用多浓度多时间处理方式。Mutation rate and mutation type are important bases for phenotype screening and constructing saturation mutant library. Different treating time and different concentrations of Ethylmethylsulfone（EMS）for BTx623 seeds have been used in this study, mutation rate and mutation type are quite different. Mutation types are very rich, including leaf color, leaf shape, spike type, fertility, and growth period. According to the emergence rate, seed rate and mutation rate, 20 h＋EMS 0.2% is considered the optimum treating time and concentration. However, if we want to build a saturated mutant library, different treating-time and various concentrations should be used.

关 键 词：高粱 甲基磺酸乙酯(EMS) 突变体 突变率 突变类型 

分 类 号：S514[农业科学—作物学]