三角褐指藻的诱变育种及产EPA的条件研究  

The Research on Mutagenesis Breeding of Phaeodactylum tricornutum and the Condition of EPA Producing

作　　者：刘红全[1] 林小园[1] 李洁琼[1] 潘艺华 

机构地区：[1]广西民族大学海洋与生物技术学院化学与生物转化过程新技术广西高校重点实验室,南宁530006

出　　处：《生物技术通报》2014年第9期114-119,共6页Biotechnology Bulletin

基　　金：国家自然科学基金项目(30960215);广西自然科学基金项目(桂科青0728019)

摘　　要：旨在获得不饱和脂肪酯EPA产量更高的藻种,利用0.6% EMS对三角褐指藻进行诱变。通过单细胞分离技术得到1株突变株EP1,其EPA产量比出发藻株提高了19.81%。结果显示,突变藻株产EPA的最适条件为:NaNO3 75 mg/L,pH7.5,昼夜温度17-15℃,接种量为12%。最适条件下培养7 d,其EPA产量可达26.77 mg/g。传代试验表明,突变藻株具有较好的遗传稳定性。In order to obtain algal strains of high EPA production, Phaeodactylum tricornutum was mutated by 0.6% EMS. The mutant strain EP1 was isolated by single cell clone from 200 mutant clones. The EPA yield of EP1 was increased by 19.81% compared with the parent strain. The most suitable foster condition includes NaNO3 75 mg/L, pH7.5, day-night temperature 17-15℃, 12% inoculation quantity and training 7 days. The EPA yield of EP1 could reach 26.77 mg/g under the suitable foster condition. The results of subculture test showed that the mutant strain possesses good hereditary stability.

关 键 词：三角褐指藻 EMS诱变 EPA 

分 类 号：Q943[生物学—植物学]