腺嘌呤和GA_3对满天星组培苗生长的影响  

Effect of Adenine and GA_3 on Growth of Gysophila paniculata Seeding

作　　者：杨春梅[1,2] 吴丽芳[1,2] 汪国鲜[1,2] 屈云慧[1,2] 曹桦[1,2] 单芹丽[1,2] 孟金贵[3] 

机构地区：[1]云南省农业科学院花卉研究所国家观赏园艺工程技术研究中心云南省花卉育种重点实验室,云南昆明650205 [2]云南云科花卉有限公司,云南昆明650205 [3]云南农业大学园林园艺学院,云南昆明650201

出　　处：《安徽农业科学》2014年第27期9279-9280,共2页Journal of Anhui Agricultural Sciences

基　　金：云南省技术创新暨产业发展专项资金项目(2012XB053)

摘　　要：[目的]探讨满天星组培苗生长适宜的GA3浓度和腺嘌呤浓度。[方法]采用云南省农业科学院花卉研究所组培中心继代培养2代的满天星品种‘小仙女’组培苗为材料,切取1 cm左右的带芽茎段为外植体,在MS+BA 0.3 mg/L+NAA 0.1 mg/L培养基中分别加入0.3、0.5、1.0、1.5、2.0 mg/L的腺嘌呤和赤霉素(GA3)进行研究。[结果]腺嘌呤、GA3具有促进满天星组培苗生长的作用,腺嘌呤适宜的使用浓度为1.5 mg/L;GA3适宜的使用浓度为1.0 mg/L。在同等条件下,腺嘌呤对满天星组培苗生长的促进作用比GA3效果好。[结论]为今后大力发展满天星组培苗奠定基础。[Objective] The aim was to discuss the suitable GA3 and ademine concentration of Gysophila paniculata seeding growth.[Method] Using 2 generations Gysophila paniculata ‘ fairies’ seeding subcultured by Tissue Culture Center of Yunnan Province Academy of Agricultural Sciences Institute as material,cutting out 1 cm long with bud stem segments as explants,MS ＋ BA 0.3 mg/L ＋ 0.1 mg/L NAA medium adding respectively 0.3,0.5,1.0,1.5,2.0 mg/L of adenine and gibberellic acid （GA3） were studied.[Result] Adenine,GA3 had the role of promoting the growth of Gysophila paniculata.The appropriate concentration of adenine was 1.5 mg/L,and the suitable concentration of GA3 was 1.0 mg/L.Under the same condition,Adenine was better than the effect of GA3 on promoting growth effect of Gysophila paniculata.[Conclusion] The study laid a foundation for developing Gysophila paniculata seeding in the future.

关 键 词：腺嘌呤 GA3 满天星 组培苗 生长 

分 类 号：S188[农业科学—农业基础科学]