益气解毒方对体外培养小鼠肝癌H22细胞生长的抑制作用  

Yiqijiedu Recipe on the Proliferation Inhibition in Ascites Hepatoma Cell Line H22

作　　者：王微[1] 马立斌[2] 尚云龙[2] 董燕飞[2] 李洪禹[2] 葛学萍 孟令玉[2] 韩泽华[2] 王冰梅[1] 

机构地区：[1]长春中医药大学基础医学院生理教研室,长春130117 [2]长春中医药大学临床医学院,长春130117 [3]长春中医药大学药学院,长春130117

出　　处：《吉林中医药》2014年第8期832-834,共3页Jilin Journal of Chinese Medicine

基　　金：国家级大学生创新创业训练计划项目(编号:201210199006)

摘　　要：目的 MTT法观察益气解毒中药复方含药血清对小鼠肝癌H22细胞生长的抑制作用。方法大鼠灌胃制备益气解毒复方中药含药血清,作用于体外培养的小鼠肝癌H22细胞,采用MTT法观察益气解毒中药复方含药血清对细胞生长的抑制作用。结果高剂量血清组与对照血清组之间的吸光度差异有显著统计学意义(P<0.001),随着作用时间的延长,其吸光度逐渐降低,抑制率逐渐增高。结论益气解毒中药复方含药血清对小鼠肝癌H22细胞生长具有较好的抑制作用。Objective To observe the inhibitory effect on H22 tumor cells by Yiqijiedu recipe containing serum by MTF assay. Methods Be preparated Yiqijiedu recipe containing serum by gavage to rats and to effect on cultured mouse hepatoma H22 cells. Inhibitory effect on cell growth was observed by MTF assay. Results The difference between the absorbance of the high-dose group and control sera sera group was statistically significant （P 〈 0. 001 ）. With the extension of time, the absorbance decreased, the inhibition rate was gradually increased. Conclusion A Yiqijiedu recipe containing serum on the growth of tumor cells in mice with better inhibition.

关 键 词：肝癌 益气解毒 含药血清 H22细胞 生长抑制率 小鼠 

分 类 号：R285.5[医药卫生—中药学]