热毒宁注射液体外抑制甲型H1N1流感病毒的研究  

In vitro inhibition of Reduning Injection on influenza A/H1N1 influenza virus

作　　者：孙兰[1,2] 段书敏[3] 周军[1,2] 王振中[1,2] 毕宇安[1,2] 萧伟[1,2] 

机构地区：[1]江苏康缘药业股份有限公司,江苏连云港222001 [2]中药制药过程新技术国家重点实验室,江苏连云港222001 [3]中国疾病预防控制中心病毒病预防控制所,北京102206

出　　处：《现代药物与临床》2014年第8期848-851,共4页Drugs & Clinic

基　　金：国家重点基础研究发展计划(973计划)项目(2010CB735604)

摘　　要：目的研究热毒宁注射液体外抑制甲型H1N1流感病毒的作用。方法以奥司他韦为阳性对照,采用CPE和MTT法观察热毒宁注射液对甲型H1N1流感病毒的抑制作用。结果 CPE法结果表明热毒宁注射液最大无毒浓度(TC0)为16.2mg/mL,半数中毒浓度为(TC50)为(24.5±8.1)mg/mL;MTT法测定结果表明热毒宁注射液TC0为16.2 mg/mL,TC50为(21.7±9.4)mg/mL。热毒宁注射液体外抑制甲型H1N1流感病毒结果显示,CPE法和MTT法热毒宁注射液作用感染细胞1次组半数有效浓度(IC50)为(900.0±173.2)、(933.3±57.7)μg/mL,治疗指数(TI)为27.2、23.2;热毒宁注射液作用感染细胞3次组IC50为(666.7±115.5)、(866.7±208.1)μg/mL,TI为36.7、25.0。结论热毒宁注射液具有明显体外抗甲型H1N1流感病毒的作用。Objective To study the inhibitory effects of Reduning Injection on influenza A/H1N1 influenza virus in vitro. Methods With Oseltamivir as positive control, CPE and MTT methods were used to observe inhibitory effects of Reduning Injection on influenza A/H1Nl influenza virus in vitro. Results The CPE method results showed that maximum of no toxicity concentration （TC0） of Reduning Injection was 16.2 mg/mL, and median toxic concentration （TC50） was （24.5 ± 8.1） mg/mL. The MTT method results showed that maximum of no toxicity concentration （TC0） was （16.2 ± 0） mg/mL, and median toxic concentration （TC50） was （21.7 ±9.4） mg/mL. The in vitro inhibition of influenza A/H1N1 influenza virus of Reduning Injection showed the. administration of Reduning Injection to infected cells once, median toxic concentration （TC50） of the CPE method and the MTT method were （900.0 ± 173.2） μg/mL, （933.3 ± 57.7） μg/mL, therapeutic indexes （TI） were 27.2 and 23.2, and administration of Reduning Injection to infected cells for three times, median toxic concentration （TC50） of the CPE method and the MTT method were （666.7 ± 115.5） μg/mL, （866.7 ± 208.1） μg/mL, TI were 36.7 and 25.0. Conclusion Reduning Injection has obvious resistance to the influenza A/H1N1 influenza virus in vitro. Administration of Reduning Injection to infected MDCK cells for three times is superior to the administration of Reduning Injection to infected MDCK cells once.

关 键 词：热毒宁注射液 流感病毒 甲型H1N1 

分 类 号：R285.5[医药卫生—中药学]