HPLC-ELSD法测定中药两头尖中竹节香附素A  

Determination of raddeanin A from Anemone raddeana Rhizoma by HPLC-ELSD

作　　者：张彦飞[1] 刘丹[1] 张蕾[1] 李连坤[1] 贡济宇[1] 蔡广知[1] 

机构地区：[1]长春中医药大学,吉林长春130117

出　　处：《中成药》2014年第9期1930-1932,共3页Chinese Traditional Patent Medicine

摘　　要：目的建立两头尖中竹节香附素A的HPLC-蒸发光散射检测器（ELSD）测定方法。方法两头尖乙醇提取物的分析在Agilent 300 Extend-C18色谱柱（4.6 mm×100 mm,3.5μm）实施,流动相为乙腈-水（0.1%甲酸）（43∶57）,体积流量1 mL/min,柱温30℃。结果竹节香附素A在0.309~2.060μg范围内线性关系良好,r=0.999 4,平均回收率为97.9%,RSD为1.4%。12个不同产地的两头尖中竹节香附素A含有量为0.11%~0.34%之间,多数地区所产药材能够达到药典要求。结论建立了两头尖中竹节香附素A的ELSD检测方法,它的定量限较UV降低了10倍,可推荐作为定量测定方法。AIM To establish a method of determining raddeanin A from Anemone raddeana Rhizoma by HPLC- ELSD. METHODS The analysis of ethanolic extract of A. raddeana Rhizoma was performed on Agilent 300 Extend-Cls Column （4.6 mm× 100 mm, 3.5 μm） of HPLC- ELSD with acetonitrile-water （0. 1% formic acid） （43 ： 57） as mobile phase, 1 mL/min of flow rate and 30 ℃ of column temperature. RESULTS There was a good linear relationship in the range of 0. 309 - 2. 060 μg for raddennin A （ r = 0. 999 4）, the average recov- ery was 97.9% , and RSD was l. 4%. Raddeanin A from twelve growing areas fell within 0. 11% 4）. 34% , most of which came up to China＇ s pharmacopoeia. CONCLUSION The limit test of established ELSD method for rad- deanin A from A. raddeana Rhizoma is ten times less than that of UV, and it is recommended as the quantitative standard for this herb.

关 键 词：两头尖 竹节香附素A HPLC-ELSD 

分 类 号：R284.1[医药卫生—中药学]