甜菜碱型两性表面活性剂对细胞的增殖抑制作用  

Proliferation inhibition effect of two kinds of betaine amphoteric surfactants on cells

作　　者：段见 何峰[1] 王晓宁[1] 廖青[1] 

机构地区：[1]北京服装学院材料科学与工程学院,北京100029

出　　处：《纺织学报》2014年第9期79-84,共6页Journal of Textile Research

基　　金：北京市教委2013年度创新能力提升计划项目(TJSHS201310012002)

摘　　要：为研究甜菜碱型两性表面活性剂结构与细胞毒性之间的关系,通过噻唑蓝(MTT)比色法检测了4种单长链烷基二甲基丙磺基甜菜碱和3种单长链烷基二甲基羟丙基磺基甜菜碱两性表面活性剂对中华仓鼠卵巢细胞(CHO)、人宫颈癌细胞(HeLa)及人体皮肤成纤维细胞(CCC-ESF-1)的细胞毒性。结果显示:这2类甜菜碱型两性表面活性剂的浓度越高,其细胞毒性越大;而且脂肪链长度越长,其细胞毒性越大。单长链烷基二甲基丙磺基甜菜碱的细胞毒性低于相同脂肪链长度的单长链烷基二甲基羟丙基磺基甜菜碱。In order to study the relationship between the structure and cytotoxicity of the amphoteric surfactants, the cytotoxicity of betaine amphoteric surfactants, including： four alkyl dimethyl propyl sulphobetaines and three alkyl dimethyl hydroxypropyl sulphobetaines on HeLa cells, Chinese Hamster Ovary （CHO） cells and human skin fibroblast （CCC-ESF-1） cells is assayed by MTT. The results indicated that the cytotoxicity of the chemicals on the cells above gradually becomes larger with their concentration increased. Proliferation inhibition effect of all the compounds increases with the growing in number of carbons. Cytotoxicity of alkyl dimethyl propyl sulphobetaine is lower than that of alkyl dimethyl hvdroxyproovl sulphobetaine.

关 键 词：单长链烷基二甲基丙磺基甜菜碱 单长链烷基二甲基羟丙基磺基甜菜碱 中华仓鼠卵巢细胞 人宫颈癌细胞 人体皮肤成纤维细胞 噻唑蓝比色法 

分 类 号：TS102.51[轻工技术与工程—纺织工程]