掌叶木种子萌发过程中过氧化物酶和超氧化物歧化酶同工酶分析  

Analyse of Peroxidase and Superoxide Dismutase Isozymes During Seed Germination on Handeliodendron bodinieri(Levl.)Rehd.

作　　者：李磊[1] 李雪萍[2] 郭松[1,3] 彭健[1] 李在留[1,4] 

机构地区：[1]广西大学林学院,广西南宁530004 [2]河南科技大学林学院,河南洛阳471003 [3]南京林业大学 江苏省林业生态工程重点实验室,江苏南京210037 [4]华中农业大学园艺林学学院,湖北武汉430070

出　　处：《北方园艺》2014年第17期102-105,共4页Northern Horticulture

基　　金：国家自然科学基金资助项目(31060053);广西自然科学基金资助项目(2011GXNSFB018016);河南省自然科学基金资助项目(62580021)

摘　　要：以掌叶木种子为试材,应用聚丙烯酰胺凝胶垂直平板电泳法,对掌叶木去除种皮的种子萌发过程中过氧化物酶（POD）和超氧化物歧化酶（SOD）同工酶动态变化进行了研究。结果表明：在掌叶木种子萌发初期（0~2d）,种子吸水打破休眠而合成少数POD、SOD同工酶;萌发中期（3~5d）,种子开始萌动,胚根逐渐伸长,SOD和POD 2类同工酶均有新酶带合成,2类酶的种类、含量迅速增加,标志着种子开始萌发;萌发后期（6~9d）,胚根继续伸长,子叶逐渐展开,胚芽开始生长,SOD、POD 2类同工酶酶含量有所减弱,但各同工酶的种类不减,仍有新酶合成。不同萌发时期同工酶的变化可作为掌叶木种子萌发各阶段的重要标志。Taking the seeds of Handeliodendron bodinieri（Lévl.）Rehd.as materials,vertical slab polyacrylamide gel electrophoresis method was used to study the dynamic change of peroxidase（POD）and superoxide dismutase（SOD）during the germination of Handeliodendron bodinieri seeds which had removed the seed coat.The results showed that,in the early seed germination period（0~2d）,the dormant state of seeds was broken and absorbed water to synthesize a few POD and SOD enzymes.In the middle（3~5d）,seeds began to germinate,radicle elongated,new bands of SOD and PODisozymes were synthesized,content increased rapidly,marked the seeds begin to germinate.In the later stage（6~9d）,radicle continued elongating,cotyledon unfolded gradually,embryo began to grew,SOD and POD isozymes content decreased,but the type of each isozyme unabated,new enzyme was synthesized.The changes of those isozymes could be used as an important sign in each stage of Handeliodendron bodinieri seed germination.

关 键 词：掌叶木 过氧化物酶同工酶(POD) 超氧化物歧化酶同工酶(SOD) 种子萌发 

分 类 号：Q949.755.5[生物学—植物学]