转鞭毛蛋白基因水稻细菌性条斑病抗性研究  

Resistance to Xanthomonas oryzae pv.oryzicola in Transgenic Rice Introduced Flagellin Gene

作　　者：王晓宇[1] 陈志谊[1] 刘文真[2] 罗楚平[1] 张荣胜[1] 周华飞[1] 

机构地区：[1]江苏农业科学院植物保护研究所,南京210014 [2]中国水稻研究所,杭州310006

出　　处：《西北植物学报》2014年第8期1534-1539,共6页Acta Botanica Boreali-Occidentalia Sinica

基　　金：江苏省自然科学基金(BK2012782)

摘　　要：该研究从生防菌枯草芽胞杆菌Bs-916中克隆了鞭毛蛋白基因,利用转基因载体pCAMBIA1300转入水稻,筛选得到98株阳性转基因植株。分子检测结果表明,有12个转基因株系可检测到目的基因的表达。随后抗病性鉴定表明,有3个转基因株系对水稻细菌性条斑病具有较高的抗性。该研究为目前水稻抗细菌性条斑病转基因研究拓宽了可应用基因资源的范围。In this study,flagellin gene cloned from biocontrol strain Bs-916（Bacillus subtilis）was introduced into rice by transgenic vector pCAMBIA1300,98 positive transgenic lines were obtained.PCR and RT-PCR test were carried out to detect the expression of target genes and 12 transgenic lines detected expression of fla gene.Subsequently,identification of disease resistance showed that three transgenic lines enhances resistance to Xanthomonas oryzae pv.oryzicola.It is clear from this research that flagene can be used to control disease,provided a potential resistance gene for controlling bacterial leaf streak.These will shed light on their utility in crop improvement strategies.

关 键 词：鞭毛蛋白基因 水稻细菌性条斑病 水稻 枯草芽胞杆菌 

分 类 号：Q789[生物学—分子生物学]