Wzz酶对O抗原糖链聚合度的调控  

Regulation of the O-antigen polysaccharide chain length by Wzz- A review

作　　者：韩东雷 马中瑞[1] 陈敏[1] 

机构地区：[1]山东大学微生物技术国家重点实验室,国家糖工程技术研究中心,山东济南250100

出　　处：《微生物学报》2014年第9期971-976,共6页Acta Microbiologica Sinica

基　　金：国家自然科学基金项目(31270983;31070824;J1103515);山东省自然科学基金项目(2009ZRB019SQ);教育部留学回国人员科研启动基金~~

摘　　要：革兰氏阴性细菌表面的O抗原在信号识别、黏附、免疫逃避等过程中发挥着重要作用。根据翻转酶的不同,O抗原的合成可划分为3种机制,其中依赖于Wzy的合成途径(Wzy-dependent)较为普遍。该机制中,wzz基因参与调节O抗原多糖的聚合度。O抗原糖链能够影响致病菌的抗原性并不同程度的刺激免疫系统。基于Wzz的蛋白晶体结构,对wzz基因进行分子改造可以使菌株合成不同大小的O抗原糖链。利用病原菌的O抗原与特定载体蛋白结合而成的糖缀合物疫苗,既具有很好的靶向性,又有较强的免疫原性。因此了解Wzz(调节糖链长短的蛋白)的功能、结构及作用机制对今后糖缀合物疫苗的开发与生产具有重要意义。The O-antigen of Gram-negative bacterium plays an important role in the signal identification, adhesion, immune evasion and other processes. There are three O-antigen polysaccharides biosynthesis mechanisms according to the type of the flippase that is involved. The Wzy-dependent mechanism is more commonly seen. In Wzy-dependent mechanism, the wzz gene is involved in regulating the length of O-antigen polysaccharide chain which can affect antigenicity of the pathogen and immune response of the host. Based on the crystal structure of Wzz （ regulator of the O- antigen polysaccharides length） , different length of O-antigen chain can be obtained through molecular modification of the gene wzz. Conjugating O-antigen or its mutants of a pathogen to a carrier protein could help to develop a vaccine that have both a good target specificity and a strong immunogenicity. Therefore, it is important to understand the function, structure and mechanism of Wzz for the development and production of glycoconjugates vaccine.

关 键 词：O抗原 Wzz(调节糖链长短的蛋白) 重复糖单位 

分 类 号：Q78[生物学—分子生物学]