登革病毒包膜蛋白的原核表达及免疫原性研究  

In vitro expression and characterization of dengue virus envelope protein

作　　者：房树玉[1] 吴艳花[1] 崔晓云[1] 范东瀛[1] 安静[1] 

机构地区：[1]首都医科大学基础医学院病原生物学系微生物学教研室,北京100069

出　　处：《微生物与感染》2014年第3期152-156,共5页Journal of Microbes and Infections

基　　金：国家自然科学基金(81271839);北京教育委员会科技计划项目(SQKM201210025005)

摘　　要：登革病毒感染引起的登革热和登革出血热/登革休克综合征是目前流行最为广泛的虫媒病毒病,但其发病机制不明,也无疫苗和特异性抗病毒药物用于防治。登革病毒包膜蛋白(E蛋白)在病毒致病和免疫过程中发挥重要作用。本研究构建了登革病毒2型E蛋白基因的重组质粒E/pGEX-6P-1,并优化表达条件,获得登革病毒E蛋白的高效可溶性表达;用谷胱甘肽琼脂糖凝胶4B亲和层析柱纯化,获得纯度较高的蛋白。该蛋白具有较好的免疫原性,免疫小鼠后可获得高效价抗体。本研究为进一步了解登革病毒E蛋白的功能及其在相关疾病中的应用奠定了基础。The classical dengue fever （DF） and dengue hemorrhagic fever/dengue shock syndrome （DHF/ DSS） caused by dengue virus （DV） are the most important infectious diseases mediated by arthropod-borne viruses. The pathogenesis of DV infection is not completely clear and there is no licensed vaccine or specific antiviral drug available currently. Envelope （E） protein of DV plays an important role in pathogenesis and immunity response. In this study, the recombinant E/pGEX-6P-1 plasmid expressing E protein of DV serotype 2 （DV2） was constructed. After optimizing the expression conditions, including the optimum temperature and incubation time, high purity of E protein could be obtained and it could induce high titre of specific antibodies against DV2 in BALB/c mice. This study provided useful information to further understand the function and application of DV E protein.

关 键 词：登革病毒 包膜蛋白 原核表达 免疫原性 

分 类 号：R373.33[医药卫生—病原生物学]