Scirpusin A和scirpusin B体外抗人类免疫缺陷病毒1型的作用  

Anti-human immunodeficiency virus type 1 activity of scirpusin A and scirpusin B in vitro

作　　者：朱景玙 杨国勋[4] 孟哲峰[1,2,3] 徐建青[1,2,3] 张晓燕[1,2,3] 

机构地区：[1]上海市(复旦大学附属)公共卫生临床中心,上海201508 [2]复旦大学生物医学研究院,上海200032 [3]复旦大学基础医学院教育部/卫生部医学分子病毒学重点实验室,上海200032 [4]复旦大学药学院,上海201203

出　　处：《微生物与感染》2014年第3期157-162,共6页Journal of Microbes and Infections

基　　金：国家自然科学基金(81261120388;81171553);"十二五"国家科技重大专项(2012ZX10001-007-008)

摘　　要：Scirpusin A和scirpusin B是从药用植物中发现的2种天然茋类二聚体,具有一定的抗病毒效应。本研究利用人类免疫缺陷病毒1型(HIV-1)包膜和水疱性口炎病毒G蛋白(VSV-G)包膜的HIV假病毒及实验室适应株HIV-1ⅢB,在体外评价2种药物的抗病毒效果并初步探讨其作用机制。研究发现,scirpusin A和scirpusin B不仅能在体外有效抑制HIV假病毒感染TZM-bl细胞(一种HIV-1易感细胞),还能抑制实验室适应株HIV-1ⅢB。Scirpusin B的作用优于scirpusin A。对实验室适应株HIV-1ⅢB,scirpusin B的半数抑制浓度(IC50)为1.33μmol/L,scirpusin A的IC50为4.77μmol/L。此外,scirpusin A和scirpusin B还能抑制VSV-G包膜假病毒,提示其作用可能与病毒在细胞内的复制过程相关。Scirpusin A在病毒进入细胞后仍可发挥抑制作用,但具体机制尚待深入研究。Scirpusin A and scirpusin B, stilbene dimers purified from medicinal plants, showed potent antiviral activity. In this study, human immunodeficiency virus （HIV） envelope and vesicular stomatitis virus G protein （VSV-G）-pseudotyped HIV-1 as well as laboratory-adapted HIV-1 strain ⅢB were used to evaluate the effects of these two compounds against HIV-1 in vitro. The results indicated that scirpusin A and scirpusin B prevented infection of TZM-bl cell line （susceptible to HIV-1 infection） by pseudotyped HIV-1 and laboratory strain Ⅲ B in vitro. Seirpusin B showed a little higher activity than scirpusin A. The 50% inhibitory concentration （IC50） of scirpusin B was 1.33 μmol/L while the IC50 of scirpusin A was 4.77 μmol/ L. Scirpusin A and scirpusin B also showed similar effects on inhibiting VSV-G pseudotyped HIV-1 in TZM-bl cells, suggesting these two compounds can affect the cellular replication process of HIV-1. The preliminary study indicates that scirpusin A could play antiviral roles after virus entering cell. There is still a long way to go to investigate the detailed mechanism.

关 键 词：人类免疫缺陷病毒1型 Scirpusin A Scirpusin B 茋类化合物 中药 水疱性口炎病毒G蛋白 

分 类 号：R512.91[医药卫生—内科学]