氯化锂对人骨髓间充质干细胞迁移的影响  

Effect of Lithium chlorid on Human Mesenchymal Stem Cells Migration

作　　者：周鼎[1] 朱振中[1] 殷俊辉[1] 翁诗阳[1] 张长青[1] 

机构地区：[1]上海交通大学附属第六人民医院骨科,上海200233

出　　处：《现代生物医学进展》2014年第27期5207-5209,共3页Progress in Modern Biomedicine

基　　金：国家自然科学基金项目(81200944/H0910);中国博士后基金项目(2012M520044)

摘　　要：目的:探究氯化锂(Lithium chlorid,LiCl)对人骨髓间充质干细胞(human Mesenchymal Stem Cells,hMSCs)迁移的影响。方法:采用划痕试验、Transwell chamber等方法,在梯度浓度LiCl作用下,观察对hMSCs迁移效果的影响并进行分析。结果:1划痕试验显示hMSCs在梯度浓度LiCl作用下,细胞迁移距离逐渐减少,差异具有统计学意义(P<0.05)。2 Transwell chamber实验显示hMSCs在梯度浓度LiCl作用下,穿梭至小室下方的细胞逐渐减少,锂剂作用组迁移细胞数差异与对照组比较有统计学意义(P<0.05)。结论:LiCl可抑制hMSCs的迁移且呈浓度依赖性。Objective： To study effect of lithium chlorid（LiCl） on human mesenchymal stem cells（hMSCs） migration. Methods：Using scratch assay and Transwell chamber methods, the hMSCs migration was detected and analyzed after different concentration of lithium. Results： 1 The scratch assay results showed that the migration distance decreased with the dose escalation of LiCl. There is statistical significance between all experimental groups and control group（P〈0.05）. 2 The Transwell chamber results showed that the cell number which transited to downside of the chamber decreased with the dose escalation of LiCl. There is statistical significance between all experimental groups and control group（P〈0.05）. Conclusion： hMSCs migration can be inhibited by LiCl, which displays a concentration dependence trend.

关 键 词：氯化锂 人骨髓间充质干细胞 细胞迁移 

分 类 号：Q95-3[生物学—动物学] R54[医药卫生—心血管疾病]