外周血单个核细胞计数与2型糖尿病的相关性分析  

Correlation Analysis between Peripheral Blood Mononuclear Cell Numbers and Type 2 Diabetes Mellitus

作　　者：魏红霞[1] 宋宏岩[1] 张葵[1] 李雷[1] 谭婷婷[1] 张正芳[1] 钱世宁[1] 

机构地区：[1]南京大学医学院附属鼓楼医院检验科,江苏南京210008

出　　处：《现代生物医学进展》2014年第27期5286-5289,共4页Progress in Modern Biomedicine

基　　金：南京市青年科技人才启动项目资助(QYK10145)

摘　　要：目的:将传统的外周血单个核细胞分离计数方法加以改进,并探讨2型糖尿病(DM)患者外周血单个核细胞数量与疾病的相关性。方法:密度梯度离心分离30例健康体检者单个核细胞,不同染色稀释液重悬细胞,充入改良牛鲍计数板连续计数5天,计算批内、批间变异;采用上述方法分离2型DM患者抗凝全血29例,计算单个核细胞数量;采用全自动生化分析仪检测对照组和2型DM组患者血脂。结果:两组的单个核细胞经3种前处理染液稀释重悬细胞后,计数结果间无统计学差异(P>0.05);2型DM组与健康对照组比较,外周血单个核细胞计数绝对值无显著性差异(P>0.05),血清TG、TC、LDL水平明显升高,差别具有统计学意义(P<0.05),而其他指标结果间比较无差异。在对照组中,单个核细胞计数结果与白细胞呈正相关(r=0.611,P<0.05),与血清TC、LDL水平呈负相关(r1=-0.550,r2=-0.605,P<0.05)。而T2DM组,单个核细胞计数结果仅与LDL水平呈负相关(r=-0.104,P<0.05)。结论:外周血单个核细胞数与2型糖尿病无明显相关性。Objective： To improve traditional counting method of peripheral blood mononuclear cells, and investigate association between quantity of peripheral blood mononuclear cell and type 2 diabetes mellitus （DM）. Methods： Mononuclear cells of 30 healthy people were obtained by density gradient centrifugation, mixing with different colored dilution and counting in continuous 5 days, establishing interassay variation and intraassay coefficient of variation;Peripheral blood mononuclear cell of 29 type 2 diabetic patients were gained by above-mentioned method; Plasma lipids were measured by 7600-020E automatic biochemistry analyzer. Results： Mononuclear cells of healthy people were mixed by staining solutions, there is no statistics significance among counted numbers （P 〉 0.05）; Comparing with healthy control, there is no statistics Significance between absolute value of Mononuclear cells of type 2 diabetic patients （P〉0.05）, serum TG, TC, LDL were higher obviously, with statistics significance（P〈0.05）, there is difference among results of other index. In control, counted numbers of mononuclear cells were positive correlation with leucocyte （r=0.611,P 〈0.05 ）, negative correlation with TC, LDL （r1=-0.550, r2=-0.605, P 〈0.05）. In type 2 diabetic group, counted numbers of mononuelear cells was only negative correlation with LDL （r=-0.104, P 〈0.05）. Conclusion： Numbers of mononuclear cells is no obviously association with type 2 diabetic patients.

关 键 词：单个核细胞计数 2型糖尿病 

分 类 号：R54[医药卫生—心血管疾病] R587.1[医药卫生—内科学]