LOX-1过表达诱发H9C2心肌细胞肥大  

Lectin-Like Oxidized LDL Receptor-1 Overexpression Induced H9C2 Cardiomyocyte Hypertrophy

作　　者：刘彬[1] 张竞之[1] 刘少军[1] 邱怀娜[1] 郭景新[1] 刘慰华[1] 

机构地区：[1]广州医科大学附属第二医院 广州心血管疾病研究所,广东广州510260

出　　处：《现代生物医学进展》2014年第29期5644-5647,共4页Progress in Modern Biomedicine

基　　金：国家自然科学基金项目(81302892);广东省自然科学基金项目(S2013040016226);中国博士后基金(2013M530363);广州医学院博士;留学归国人员基金项目(2012C50)

摘　　要：目的:构建携带过表达大鼠凝集素样氧化低密度脂蛋白受体-1(LOX-1)基因的慢病毒载体,研究LOX-1与心肌细胞肥大的关系。方法:构建大鼠LOX-1基因pHIV-LOX-1过表达质粒,与包装质粒psPAX2、pMD2G共转染293T细胞,检测其侵染效率。包装慢病毒并侵染H9C2心肌细胞,72 h后观察其侵染效率。qPCR法检测细胞LOX-1表达。检测过表达LOX-1后心肌细胞面积及其蛋白含量变化。结果:成功构建过表达LOX-1 H9C2心肌细胞。过表达LOX-1组(Lv.LOX-1+)心肌细胞面积(16691.890±1022.368μm2)较对照组(Lv.NC)(3459.865±343.175μm2)显著增加(P<0.001)。Lv.LOX-1+组心肌细胞蛋白含量(132.457±8.188 pg/cell)较Lv.NC组(45.095±1.655 pg/cell)显著增加(P<0.001)。结论:LOX-1过表达能诱导H9C2心肌细胞肥大。Objective： To construct the lentiviral overexpression vector of Lectin-like oxidized LDL receptor-1 （LOX-1） and investigate its correlation with cardiomyocyte hypertrophy in H9C2 Cells. Methods： The lentiviral vector was constructed by inserting the lentiviral vectors with the LOX-1 gene fragment. The recombination lentiviral particles were produced by the packaging 293T cell. The culture supematant was harvested and the titration of them was calculated by the limiting dilution. H9C2 cells were infected with recombinant lentivirus overexpressing LOX-1 for 72 hours, then transduction efficiency was investigated and LOX-1 mRNA level in cells was examined by the qPCR. The cell size was measured by immunofluorescence. The cell protein content was examined by BCA kit. Results： Overexpressing LOX-1 H9C2 cardiomyocytes were constructed successfully. The cell size of LOX-1 overexpression group （Lv. LOX-1＋）（ 16691.890 ± 1022.368 μm2） was remarkably increased compared with control gruop （Lv.NC）（3459.865±343.175μm2） （P〈0,001）. The protein content was enhanced after LOX-1 overexpression （132.457± 8.188 pg/cell） compared with control group （45.095±1.655 pg/ceU）（P〈0.001）. Conclusion： LOX-1 overexpression can induce cardiomyocytes hypertrophy in H9C2 cells.

关 键 词：凝集素样氧化低密度脂蛋白受体-1 心肌细胞肥大 慢病毒载体 

分 类 号：R541[医药卫生—心血管疾病] R541.3[医药卫生—内科学]