液相色谱法同时测定防晒霜中16种紫外线吸收剂  

Determination of 16 UV-Filters in Sunscreens by High Performance Liquid Chromatography

作　　者：陈伟[1] 周丽丽[1] 李洋[1] 陈会明[1] 王琤[1] 李蕾[1] 于文莲[1] 

机构地区：[1]中国检验检疫科学研究院,北京100123

出　　处：《检验检疫学刊》2014年第4期28-32,共5页Journal of Inspection and Quarantine

基　　金：2010年检验检疫行业标准制修订项目(2010B364r)

摘　　要：建立了同时检测防晒霜中16种紫外线吸收剂含量的高效液相色谱分析方法。样品用甲醇、四氢呋喃、高纯水、高氯酸的混合溶液溶解后超声提取。采用新型ZORBAX SB C18(5μm,250 mm×4.6 mm i.d.)色谱柱,以甲醇、四氢呋喃、高氯酸水溶液(V/V/V)(pH 2.6)梯度洗脱,检测波长311 nm。结果显示在22min内16种紫外线吸收剂完全分离,在10-4000μg/mL范围内呈线性关系,相关系数大于0.9990;方法定量限为0.02%-0.4%;在市售防晒霜中加标回收率为84.0%-113.9%,相对标准偏差为2.49%-5.75%。本方法简便准确,能够满足进出口检验的需要。A high performance liquid chromatography （HPLC） method was established simultaneous to detect the content of sixteen UV-filters in sunscreens. The samples were dissolved by methanol ＋ THF ＋Ultra-pure water ＋ HCIO4 and extracted by ultrasonic method. The target compounds were separated by a new ZORBAX Eclipse Plus C18column （5pro, 250 mmx4.6 mm i.d.） in gradient elution mode using methanol, THF and aqueous perchloric acid （pH=2.6） as mobile phase, and detected by a photo-diode array detector at 311 nm. The results indicated that the sixteen UV-filters were completely separated in 22 min. The calibration curves was showed good linearity in the range of 10-4000μg/mL, with correlation coefficients larger than 0.9990. The limits of quantitation were in the range of 0.02 to 0.4%. The spiked recoveries of commercial sunscreens were in the range of 84.0% to 113.9% with the relative standard deviation of 2.49% to 5.75%. The results indicated that the method was simple, rapid, accurate and suitable for the requirements in import and export inspection.

关 键 词：高效液相色谱 紫外线吸收剂 防晒霜 

分 类 号：TQ658.24[化学工程—精细化工] O657.72[理学—分析化学]