昆津病毒和巴马哈森林病毒双重RT-PCR检测方法的建立  

Establishment of Duplex RT-PCR of Kunjin Virus and Barmah Forest Virus

作　　者：林二妹[1,2] 白红岩[2] 孙肖红[2] 徐宝梁[2] 

机构地区：[1]河北省承德医学院,河北承德067000 [2]中国检验检疫科学研究院

出　　处：《检验检疫学刊》2014年第4期58-61,66,共5页Journal of Inspection and Quarantine

基　　金：国家质检总局公益项目(201210014)

摘　　要：[目的]建立昆津病毒和巴马哈森林病毒双重RT-PCR检测方法。[方法]利用Beacon Designer7.0软件设计引物,以人工合成昆津病毒巴马哈森林病毒E基因的片段作为模板,验证该方法的灵敏度及特异性。[结果]最低检出限:昆津病毒1.83×106 copies/μL,巴马哈森林病毒2.02×106copies/μL。[结论]建立的昆津病毒和巴马哈森林病毒双重RT-PCR检测方法具有灵敏度高、特异性好等特点,适合于昆津病毒和巴马哈森林病毒的快速检测。A duplex RT-PCR method for detection of Kunjin virus （KUNV） and Barmah Forest virus （BFV） was established. Based on the E gene segments of KUNV and BFV respectively, two twin primers were designed and synthesized, and the RT-PCR was developed. The specialty and the sensitivity were tested. The detection limits of the Kunjin virus was 1.83×106 copies / μL, and that of Barmah Forest virus, 2.02×106 copies / μL. A Duplex RT-PCR for KUNV and BFV detection was established and characterized by high sensitivity and rapidity.

关 键 词：昆津病毒 巴马哈森林病毒 RT-PCR 

分 类 号：Q939.4[生物学—微生物学]