PMA-PCR技术在植物病原细菌检疫中的应用前景  

The Applications Prospects of PMA-PCR Technology in Plant Pathogenic Bacteria Quarantine

作　　者：刘鹏[1] 牛春敬[1] 胡佳续[1] 冯洁[1] 王金成[1] 黄国明[1] 

机构地区：[1]天津出入境检验检疫局,天津300461

出　　处：《检验检疫学刊》2014年第4期72-74,18,共4页Journal of Inspection and Quarantine

基　　金：天津出入境检验检疫局科研课题(TK0030-2013)

摘　　要：介绍了PMA-PCR技术检测活菌的方法,列举了该方法在食品、医疗和环境微生物检测领域的应用研究情况,并讨论了PMA-PCR技术在植物病原细菌检疫工作中的应用前景。Propidium monoazide （PMA） is a kind of photosensitive dye which can pass through the damaged cell membrane and has high affinity with DNA molecules. The cross-linking of PMA and DNA can inhibit PCR amplification reactions. The paper describes the introduction of PMA-PCR method which detects viable cells and discusses the application prospects of the method in plant pathogenic bacteria quarantine.

关 键 词：PMA—PCR 病原细菌 检疫 

分 类 号：S413[农业科学—植物保护]