梅山猪脂多糖结合蛋白基因292G/A和1168G/A多态性及其对部分免疫指标的影响  

Polymorphism of 292G/A and 1168G/A in LBP Gene and their Effect on Partial Immune Indices in Meishan Pigs

作　　者：董文华[1] 刘颖[1] 朱世平[1] 黄雪根 杨建生 包文斌[1] 吴圣龙[1] 

机构地区：[1]扬州大学动物科学与技术学院,江苏扬州225009 [2]苏州苏太企业有限公司,江苏苏州215200 [3]昆山市梅山猪保种有限公司,江苏昆山215300

出　　处：《中国畜牧兽医》2014年第9期174-179,共6页China Animal Husbandry & Veterinary Medicine

基　　金：江苏省产学研前瞻性联合研究项目(BY2012157);江苏省科技支撑计划(农业)(BE2012330;BE2013345)

摘　　要：本试验采用PCR-RFLP方法对81头梅山猪群体进行脂多糖结合蛋白(lipopolysaccharide binding protein,LBP)基因292G/A和1168G/A位点的多态性检测,进而分析这2个位点的多态性与部分重要细胞因子之间的关系,旨在探讨能否将其作为机体一般抗病力的有效遗传标记。结果发现,LBP基因292G/A MspⅠ酶切位点和1168G/A Bsh1236Ⅰ酶切位点在梅山猪群体中均可检测到3种基因型:AA、AB和BB。遗传多态性分析表明,1168G/A位点PIC值介于0.25~0.5之间,表现为中度多态;292G/A位点PIC值小于0.25,表现为低度多态。关联分析结果显示,梅山猪LBP基因292G/A位点处,BB基因型个体的IFN-α显著高于AA和AB基因型个体(P<0.05);1168G/A位点处,3种基因型个体之间所检测的免疫指标均无显著差异(P>0.05)。因此,LBP基因292G/A突变对梅山猪的一般抗病力可能具有一定的影响,有必要对292G/A的遗传效应开展进一步分析与验证。Polymorphisms in 292G/A and l168G/A locus of lipopolysaccharide binding protein （LBP） gene in 81 Meishan pigs were detected by PCR-RFLP method, the relationships between two polymorphic sites and partial important cytokines in blood serum were analyzed in this study to provide feasibility analysis of those polymorphic sites used for further disease-resist- ant breeding of pigs. The results showed that 292G/A and l168G/A of LBP gene were digested by Msp I and Bsh1236 I , re- spectively, which could be divided into three kinds of genotypes （AA, AB and BB）. PIC value of Meishan pigs in 1168G/A ranged from 0.25 to 0.5, which displayed moderate polymorphism. While the value af 292G/A was less than 0.25, which dis- played low polymorphism. The correlation analysis indicated that IFN-a level of the BB genotype was significantly higher than that of the AA and AB genotypes in the 292G/A （P^0. 05）, while there had no significant differences in immune indices among the 3 kinds of genotypes based on the 1168G/A （P〈0.05）. To make a conclusion.. The mutation in the 292G/A of LBP gene had probably some effects on general disease resistance in Meishan pigs. It is necessary to make a further analysis and verification of the hereditary effects in LBP gene 292G/A.

关 键 词：猪 LBP基因 多态性 免疫指标 

分 类 号：S814.1[农业科学—畜牧学]