肠上皮紧密连接蛋白mRNA的表达与仔猪断奶腹泻关系的研究  

Study on Relationship between Intestinal Epithelium Tight Junction Proteins mRNA Expression and Piglets Weaning Diarrhea

作　　者：张影超 王希彪[1] 崔世泉[1] 狄生伟[1] 管森[1] 

机构地区：[1]东北农业大学动物科学技术学院,黑龙江哈尔滨150030

出　　处：《中国畜牧兽医》2014年第9期221-224,共4页China Animal Husbandry & Veterinary Medicine

基　　金：黑龙江省教育厅科学技术研究项目(12541007)

摘　　要：试验通过比较民猪和长白猪断奶后1周的腹泻状况,检测2品种仔猪断奶前后空肠、回肠和结肠紧密连接蛋白Zonula occludens-1(ZO-1)和Occludin的mRNA表达,探讨紧密连接蛋白表达与仔猪断奶腹泻之间的关系。结果显示,民猪仔猪断奶后的腹泻率和腹泻频率极显著小于长白猪(P<0.01),腹泻天数显著少于长白猪(P<0.05);民猪未断奶组仔猪回肠和结肠紧密连接蛋白ZO-1和Occludin的mRNA表达量极显著高于长白猪未断奶组(P<0.01);民猪断奶腹泻组仔猪回肠和结肠紧密连接蛋白ZO-1和Occludin的mRNA表达量显著高于断奶健康组(P<0.05)。上述结果说明,民猪仔猪断奶前肠道紧密连接蛋白mRNA的高水平表达,可能与保证肠道健康、减少腹泻的发生有关,而发生腹泻后通过增加紧密连接蛋白mRNA的表达,加快肠黏膜修复,实现腹泻的康复。所以,仔猪断奶前后紧密连接蛋白mRNA的高表达对仔猪抗断奶腹泻有重要意义。The experiment was carried out to compare diarrhea status between Min pig and Landrace pig in a week after weaning, detect two varieties piglets jejunum, ileum and colon tight proteins Zonula occludens-1 （ZO-1）and Occludin mRNA expression before and after weaning. The intention was to discuss the relationship between tight proteins expression and piglets weaning diarrhea. The results showed that Min piglets diarrhea rate, diarrhea frequency were very significantly lower than Landrace pig within one week after weaning （P〈0.01）, diarrhea days was significantly lower than Landrace pig within one week after weaning （P〈0.05） Min piglets not weaning group ileum and colon ZO-1 and Occludin mRNA expression was very significantly higher than Landrace pig not weaning group （P〈0.01） Min piglets weaning diarrhea group ileum and colon ZO-1 and Occludin mRNA expression was significantly higher than weaning health group （P〈0.05）. The results indicated that Min piglets intestinal tight junction proteins mRNA high expression before weaning might be had relation with guarantee piglets in testinal health and decrease occurrence diarrhea~ Min pig fast recover intestinal mucosa function and rehabilitation by increased tight junction proteins mRNA expression after weaning. So tight junction proteins mRNA high expression before and after wea- ning had an important significance for piglets to resist weaning diarrhea.

关 键 词：ZO-1 OCCLUDIN 民猪 长白猪 断奶腹泻 

分 类 号：S856.4[农业科学—临床兽医学]