CD4＋CD25＋调节性T细胞在血吸虫免疫逃避中的作用及机制研究  

Studies on the effect and mechanism of CD4＋ CD25＋ regulatory T calls in Schistosoma japonicum immune evasion

作　　者：唐春莲 郭思洁 杨进 祝青 刘晓宏 

机构地区：[1]武汉市武昌医院检验科,430063

出　　处：《国际免疫学杂志》2014年第5期449-452,共4页International Journal of Immunology

摘　　要：目的 探讨CD4＋ CD25＋调节性T细胞（Tregs）在日本血吸虫免疫逃避中的作用及其机制.方法 雌性BALB/c小鼠随机分成3组,即正常对照组、感染对照组和抗CD25单克隆抗体（anti-CD25 mAb）组,各感染组每只小鼠均经腹部皮肤感染日本血吸虫尾蚴40条,感染后两周anti-CD25 mAb组每只小鼠经腹腔注射anti-CD25 mAb 300 μg,其它组注射等体积的PBS,感染后5周杀鼠冲虫,计数每只小鼠虫荷.收集脾细胞及培养上清,流式细胞术检测脾淋巴细胞中CD4＋ CD25＋ Tregs百分比.双抗夹心ELISA法测定脾细胞培养上清中的γ-干扰素（IFN-γ）、IL-4、IL-5、IL-10的含量.结果 Anti-CD25mAb组虫荷（23.17 ±6.94）明显低于感染对照组[（30.17 ±5.85）,P=0.047];感染对照组脾淋巴细胞中CD4＋ CD25＋ Tregs百分比（2.68 ±0.12）％明显高于正常对照组[（1.98±0.33％）,P=0.049],而anti-CD25mAb组脾淋巴细胞中CD4＋ CD25＋ Tregs百分比（1.28±0.30）％明显低于感染对照组（P=0.000）;anti-CD25mAb组脾细胞培养上清中IFN-γ的含量（386.87±24.85） pg/mL明显高于感染对照组[（61.32±8.75） pg/mL,P=0.000],其余细胞因子组间无统计学意义.结论 anti-CD25 mAb能部分封闭CD4＋ CD25＋ Tregs后有利于机体清除日本血吸虫,其机制可能为增强Th1型免疫反应,宿主CD4＋ CD25＋ Tregs有助于日本血吸虫逃避宿主的免疫攻击.Objective To explore the effect and mechanism of CD4 ＋ CD25 ＋ Tregs in S.japonicum immune evasion.Methods Female BALB/c mice were randomly divided into normal control group,infected control and anti-CD25 mAb group.Each mouse was infected percutaneously with 40 S.japonicum cercaria.After 2 weeks infection,anti-CD25 mAb group was injected intraperitoneal with 300 μg anti-CD25 mAb each mouse.After 5 weeks infection,all mice were succumbed to measure worm burden.The percent of CD4＋ CD25 ＋ Tregs in spleen was measured with flow cytometer.The expression of gamma interferon （IFN-γ）,interleukin-4 （IL-4）,interleukin-5 （IL-5） and interleukin-10 （IL-10） in cultural suspension of splenic cells was detected by sandwich-ELISA after stimulation with ConA.Results Worm burden in anti-CD25 mAb group （23.17 ± 6.94） was extremely lower than in infected control group [（30.17 ± 5.85） pg/mL,P =0.047].Compared to those of normal [（1.98 ±0.33）,P =0.049] and infected control group [（2.68 ±0.12）,P =0.000],the ration of CD4＋ CD25＋ Tregs were extremely low in anti-CD25 mAb group （1.28 ± 0.30）.The level of IFN-γ in anti-CD25 mAb group [（386.87 ± 24.85） pg/mL,P =0.000] was extremely higher than that of infected control group （61.32 ± 8.75）pg/mL,while the levels of IL-4,IL-5 and IL-10 were not significant different among the three groups.Conclusion These results hint that CD4＋ CD25＋ Tregs contribute to the escape of S.japonicum from the host immune assault,while anti-CD25 mAb can partially block CD4＋ CD25＋ Tregs and favor the host to clear parasite by enhancing Th1-type immune response.

关 键 词：日本血吸虫 CD4+ CD25+调节性T细胞 γ-干扰素 白细胞介素-4 白细胞介素-5 白细胞介素-10 

分 类 号：R392[医药卫生—免疫学]