罗仙子提取物诱导白血病细胞凋亡作用研究  

Apoptosis induced by Chrysomyia Megacephala Fab Extracts in Human Promyelocytic Leukemia Cells

作　　者：屠凌岚[1,2] 郑晓亮[1,2] 颜冬梅[1,2] 马臻[1] 彭小英[1] 

机构地区：[1]浙江省医学科学院药物研究所,浙江杭州310013 [2]浙江省医学科学院分子医学中心,浙江杭州310013

出　　处：《中华中医药学刊》2014年第9期2237-2239,I0010-I0011,共5页Chinese Archives of Traditional Chinese Medicine

基　　金：浙江省科技厅省属科研院所专项(2007F10030);浙江省中医药科技计划项目(2006C042);浙江省医学重点学科群建设项目(XKQ-010-001);浙江省科技计划项目(2012F10005)

摘　　要：目的:观察中药罗仙子提取物体外对白血病细胞的凋亡诱导作用。方法:罗仙子提取物处理人白血病HL-60细胞24 h后,MTT法检测细胞活率;采用光学显微镜、荧光显微镜、透射电镜观察细胞形态学改变,流式细胞仪检测细胞凋亡率。结果:不同浓度罗仙子提取物可抑制HL-60细胞增殖同时诱导典型的细胞凋亡形态学变化,与对照组相比细胞凋亡率显著升高。结论:罗仙子提取可显著抑制白血病细胞增殖、诱导细胞凋亡。To observe the cell apoptosis induced by Chrysomyia Megacephala Fab extracts LXZ - 1A in human pro- myelocytic leukemia cells. Cell viability of HL- 60 cells treated with LXZ -1 A was measured by MTI＂ assay. LXZ -1A -induced cell morphological changes were observed with light microscope, fluorescence microscope and transmission e- lectron microscope. Cell apoptosis rate was determined by flow eytometric analysis. The IC50 （ 24 h） of LXZ - 1A for inhi- bition HL-60 cell proliferation was （2.26 ± 0. 17） mg/mL. The cell treated with LXZ - 1A （0.3 - 1.0 mg/mL） showed typical morphological changes of apoptosis and the apoptotic rate was 8% to 46% for 24 h. Chrysomyia Mega- cephala Fab extracts LXZ- 1A inhibits human promyelocytic leukemia ceils proliferation and induces cell apoptosis.

关 键 词：罗仙子提取物 白血病 细胞凋亡 

分 类 号：R285.5[医药卫生—中药学]