大孔吸附树脂纯化五味子木脂素的工艺考察  

Process investigation on purification of schisandra chinensis lignans by macroporous resin

作　　者：黄家富[1] 周晓东[2] 周山 

机构地区：[1]解放军第102医院药械科,213003 [2]南京军区南京总医院汤山分院药械科 [3]正大天晴药业集团股份有限公司南京研发中心

出　　处：《中国现代药物应用》2014年第19期12-16,共5页Chinese Journal of Modern Drug Application

摘　　要：目的建立五味子中五味子醇甲、五味子甲素、五味子乙素的高效液相色谱法(HPLC)分析方法 ,以此为指标成分,利用大孔树脂纯化五味子醇提液,筛选纯化五味子木脂素的最佳树脂及纯化的最佳工艺。对纯化前后HPLC色谱图进行比较,并测定五味子总木脂素的含量。方法动态上样法比较各种树脂对三种成分的吸附及解吸量来筛选最佳纯化树脂,通过测定三种成分的上样量和洗脱量来考察最佳上样工艺及洗脱工艺,对比纯化前后HPLC色谱图来研究纯化合理性,利用紫外分光光度法来测定纯化后五味子总木脂素的含量。结果最佳纯化树脂为AB-8,上样药液浓度0.128 g/ml,流速3 ml/min,径高比1∶3以上,95%乙醇洗脱。对比HPLC色谱图发现,纯化前后色谱峰并无减少。木脂素含量测定结果显示纯化后五味子木脂素的含量占固形物60%以上。结论纯化合理且必要,并且工艺简单实用,可用于工业生产。Objective To establish a method for determining concentrations of schisandrol A, deoxyschisandrin and schisandrin B from Schisandra chinensis by high performance liquid chromatography （HPLC）. To use macroporous resin for purification of schisandro] A, and to filtrate the superior resin for the purification of total lignans from schisandra chinensis and the optimum purification process. At last, to compare the results of HPLC chromatograms before and after purification, and to determinate the content of total lignans from schisandra chinensis. Method Based on results of adsorption and desorption of different resin for the three ingredients using dynamic injection method, the best purified resin was selected. Injection and elution volume of the three components above-mentioned were measured to determine the best injection and clution process. The reasonableness of the purification was examined according to the results of the HPLC chromatograms before and after the purification process. The content of total lignans from schisandra chinensis after purification was measured by UV spectrophotometry. Results The best purifying- resin was AB-8; the concentration of injection was 0.128 g/ml; the flow rate was 3ml/min; the diameter ratio was more than 1：3 and the ethanol elution was 95%. According to the HPLC chromatograms, chromatographic peak was not reduced after the purification process. The content of total lignans from schisandra chinensis accounted for more than 60% of the total solids after purification. Conclusion The purification process is reasonable, simple, practical and suitable for industrial production.

关 键 词：五味子木脂素 大孔树脂 纯化 高效液相色谱法 紫外分光光度法 

分 类 号：R284[医药卫生—中药学] O657.72[医药卫生—中医学]