小鼠腹腔持续置管引流模型骨髓髓系造血受抑制的研究  

Study on suppression of myeloid hematopoiesis in mice challenged with colon ascendens stent peritonitis

作　　者：孔雅娴[1] 栗雅杰[1] 马雅銮[2] 缪鹏[3] 曾辉[1] 

机构地区：[1]首都医科大学附属北京地坛医院传染病研究所,新发突发传染病研究北京市重点实验室,北京100015 [2]中国中医科学院中医基础理论研究所 [3]首都医科大学附属北京同仁医院普通外科

出　　处：《中华实验和临床感染病杂志（电子版）》2014年第4期1-4,共4页Chinese Journal of Experimental and Clinical Infectious Diseases(Electronic Edition)

基　　金：国家自然科学基金(No.81101251;No.81372095)

摘　　要：目的利用小鼠腹腔持续置管引流(CASP)模型探讨脓毒症对造血干细胞、髓系祖细胞的影响。方法采用腹腔持续置管引流手术制作脓毒症小鼠模型,以假手术组作为对照。术后24 h,采用流式细胞术检测骨髓中造血干细胞、髓系祖细胞比例的变化以及造血干细胞增殖的变化。结果与假手术组相比,CASP模型组以髓系细胞为主的骨髓总细胞数显著降低,骨髓造血干细胞(HSCs)扩增明显,多潜能祖细胞(MPPs)、共同髓系祖细胞(CMPs)和粒细胞-单核细胞祖细胞(GMPs)均显著减少。结论 CASP脓毒症模型小鼠髓系细胞减少可能与骨髓造血干细胞髓系分化障碍有关。Objective To investigate the effects of sepsis on hematopoetic stem cells （HSCs） and myeloid progenitos with mouse model which received colon ascendens stent peritonitis （CASP）. Methods The mouse model of sepsis was established by CASP surgery and sham operated mice were taken as control group. The proportions of HSCs and myeloid progenitors in bone marrow （BM） and the proliferation proportion of HSCs were analyzed with flow cytometer, 24 hours after the operation. Results Compared with the mice in sham group, the number of total BM cells, which mainly included myeloid cells, significantly decreased in CASP group. In addition, an expansion of septic BM HSCs was observed, whereas multipotent progenitors （MPPs）, common myeloid progenitors （CMPs） and granulocyte/monocyte progenitors （GMPs） significantly reduced. Conclusions The reduction of myeloid cells from CASP mouse model is related to block of myeloid differentiation in BM HSCs.

关 键 词：腹腔持续置管引流 脓毒症 应急造血 造血干祖细胞 分化障碍 

分 类 号：R459.7[医药卫生—急诊医学]