草苁蓉乙醇提取物对NAFLD大鼠肝脏炎性因子表达的影响  

Impact of boschniakia rossica ethanol extract on hepatic expression of inflammatory cytokines in rats with NAFLD

作　　者：何松美[1] 刘霞[2] 吴煜良[3] 刘旭明[1] 殷思纯[1] 龙尧[4] 

机构地区：[1]广东省东莞市人民医院感染病科,523008 [2]广东医学院基础学院生理科学实验室 [3]广东省东莞市人民医院肿瘤科,523008 [4]广东医学院附属医院感染病科

出　　处：《实用肝脏病杂志》2014年第5期519-522,共4页Journal of Practical Hepatology

摘　　要：目的探讨草苁蓉乙醇提取物(BREE)对非酒精性脂肪性肝病(NAFLD)大鼠肝组织羟脯氨酸和炎性因子的影响。方法采用高脂饲料喂养构建大鼠NAFLD模型,大鼠被随机分为正常对照组、NAFLD模型组和BREE治疗组(500 mg·kg-1·d-1)。在用药8 w末,处死动物,留取血清,采用ELSIA法检测肝组织白细胞介素(IL)-1β、肿瘤坏死因子(TNF)-α和白细胞介素(IL)-6水平;采用HE染色和免疫组化法分别观察大鼠肝脏病理学变化和检测肝组织NF-kB/p65表达。结果 BREE治疗组大鼠血清ALT[(39.47±20.26)U/L]和AST[(46.48±18.52)U/L]水平较模型组显著下降[(79.32±19.05)U/L和(88.35±17.46)U/L,P<0.01],而血清还原性谷胱甘肽[(349.43±45.52)mg/L]水平较模型组明显升高[(265.38±28.57)mg/L,P<0.01];BREE治疗组大鼠肝组织NF-kB/p65阳性表达[(15.49±4.78)%]和羟脯氨酸含量[(14.28±3.08)mg/g]均较模型组显著下降[分别为(87.54±6.59)%和(35.47±4.53)mg/g,P<0.01],同样,IL-1β、TNF-α和IL-6水平[分别为(34.51±5.61)pg/mL、(45.37±7.03)pg/mL)和(18.98±5.04)pg/mL]均较模型组显著降低[分别为(78.25±6.51)pg/mL、(85.64±6.25)pg/mL、(29.19±4.82)pg/mL,P<0.01];BREE处理组动物肝组织病理学损害程度明显减轻。结论 BREE能明显改善NAFLD大鼠肝功能和肝组织胶原沉积,其机制可能与通过下调NF-κB/p65和炎症细胞因子表达有关。Objective To investigate the effect of boschniakia rossica ethanol extract（BREE） on hydroxyproline（Hyp） and inflammatory cytokines in rats with non-alcoholic fatty liver diseases（NAFLD）.Methods The model of NAFLD in rats was established by hight-fat diet administration. Rats were randomly divided into control,model and BREE treatment group（500 mg·kg^-1·d^-1）. All rats were sacrificed to harvest serum and liver tissues at the end of eighth week. Serum levels of AST,ALT and reduced glutathione（GSH） were measured,and the levels of interleukin（IL）-1β,tumor necrosis factor（TNF）-α and IL-6 in liver tissues were detected by ELSIA. HE staining and immunohistochemistry were performed for evaluation of liver pathological changes and the expression of NF-kB/p65 in liver tissues,respectively. Results Serum levels of ALT [（39.47±20.26） U/L] and AST [（46.48 ± 18.52） U/L] in rats subjected to BREE treatment significantly decreased as compared to in the model group [（79.32±19.05）U/L and（88.35±17.46） U/L,respectively,P〈0.01];Serum GSH levels in BREE-treated rats [（349.43±45.52） mg/L] significantly increased than in the model [（265.38±28.57）mg/L,P〈0.01];The positive rates of NF-kB/p65 [（15.49±4.78）%] and Hyp contents [（14.28±3.08） mg/g] in liver tissues in BREE-treated rats significantly decreased than in the model [（87.54±6.59）% and（35.47±4.53）mg/g,respectively,P〈0.01];the IL-1β,TNF-α and IL-6 levels [（34.51±5.61） pg/mL,（45.37±7.03） pg/mL,（18.98±5.04）pg/mL,respectively] in liver tissues in BREE-treated rats were significantly lower than in the model [（78.25±6.51）pg/mL,（85.64 ±6.25） pg/mL,（29.19 ±4.82） pg/mL,respectively,P 0.01];BREE also significantly alleviated pathological changes in the liver. Conclusions BREE significantly improves liver function and collagen deposition in rats with NAFLD by down regulation of NF-κB/p65 and inflammatory cytokines in the liver.

关 键 词：非酒精性脂肪性肝病 草苁蓉乙醇提取物 核转录因子ΚB P65 炎性细胞因子 

分 类 号：R285.5[医药卫生—中药学]