HPLC法测定小裂芽梅衣中水杨嗪酸的含量  

Determination of Salazinic Acid in Parmelia nimandairana by HPLC

作　　者：李志东[1] 李涛[2] 朱晓健[3] 葛朝晖[3] 张海娟[3] 

机构地区：[1]广州中医药大学,广东广州510006 [2]浙江海洋学院海洋科学与技术学院,浙江舟山316000 [3]临沂大学山东省鲁南中药材资源开发工程技术研究中心,山东临沂276000

出　　处：《现代中药研究与实践》2014年第4期25-27,共3页Research and Practice on Chinese Medicines

基　　金：临沂大学校内资助课题(2012);批号:4613037

摘　　要：目的建立测定地衣植物小裂芽梅衣中水杨嗪酸含量的方法。方法采用HPLC法,以Inertsil ODS-3 C18(4.6 mm×250 mm,5μm)为色谱柱,甲醇-水-乙酸(45:29:1)为流动相,流速1.0mL·min-1,检测波长330 nm,柱温为40℃。结果该成分在25-200μg·mL-1浓度范围内与峰面积呈良好的线性关系(r=0.9995),平均回收率为98.2%,RSD为2.03%(n=6)。结论本方法简便可行、重复性好,可作为小裂芽梅衣的质量评价的方法之一。Objective To establish a method for the determination of salazinic acid in Parmelia nimandairana. Methods Using HPLC with Inertsil ODS-3 column （4.6 mm×250 mm, 5 μm）, mobile phase wasmethanol-water-acetic acid（45：29：1） at a flow rate of 1.0 mL·min^-1, and the detection wavelength was 330 nm, thecolumn temperature was 40℃. Results The linearity of the calibration curve was evaluated over the amount rangeof 254200 pg·mL^-1 for salazinic acid and good linearity with correlation coefficient （r） of 0.999 5 was obtained.The recovery and RSD were 98.2% and 2.03% （n=6）, respectively. Conclusion The method was simple, feasibleand reproducible, which can be used for the quality control of Parmelia nimondoiroms.

关 键 词：水杨嗪酸 小裂芽梅衣 高效液相色谱法 

分 类 号：R927.2[医药卫生—药学]