高效液相色谱-蒸发光散射检测器法测定复方桔梗止咳片中桔梗皂苷D含量  

Content Determination of Platycodin D Content in Compound Radix Platycodi Anticough Tablets by HPLC- ELSD

作　　者：陈国宁 邰顺章 黄柳燕[3] 

机构地区：[1]江苏省盐城市第二人民医院,江苏盐城224003 [2]江苏省盐城市药品检验所,江苏盐城224002 [3]盐城师范学院,江苏盐城224002

出　　处：《中国药业》2014年第18期51-53,共3页China Pharmaceuticals

摘　　要：目的建立测定复方桔梗止咳片中桔梗皂苷D含量的高效液相色谱－蒸发光散射检测器（HPLC—ELSD）法。方法色谱柱采用Agilent C18柱（150mm×4．6mm，5μm），以乙腈-水（25：75）为流动相，流速为0．7mL／min；ELSD检测器漂移管温度为90℃，载气1．8Bar；进样量为10μL；柱温为30℃。结果桔梗皂苷D质量浓度线性范围为125．08—1250．8mg／L（r=0．9998），平均加样回收率为98．60％，RSD=1．00％（n=6）。结论该方法简便、重复性好，可用于测定复方桔梗止咳片中桔梗皂苷D的含量。Objective To establish a quantitative method for the determination of platycodin D content in Compound Radix Platycodi Anticough Tablets by high performance liquid chromatography coupled with ELSD（HPLC-ELSD）.Methods The Agilent C18 column（150mm×4.6mm,5μm） was adopted. The mobile phase consisted of acetonitrile-water（25：75） at a flow rate of 0. 7 mL/min,ELSD detector was used,the drift tube temperature was 90 % ,the carrier gas 1. 8 Bar;the sample quantity： 10 μL;the column temperature 30 ℃. Results The mass concentration curve showed a good linearity in the ranges of 125.08-1 250.8 mg/L（r=0.999 8）.The average recovery rate was 98.60%, RSD = 1.00% （n=6）. Conclusion The method is simple and reproducible,which can be used for the quantitative determination of platycodin D in Compound Radix Platycodi Anticough Tablets.

关 键 词：复方桔梗止咳片 桔梗皂苷D 高效液相色谱-蒸发光散射检测器 含量测定 

分 类 号：R284.1[医药卫生—中药学] R286.0[医药卫生—中医学]