过碳酸钠和微波对食用菌种植常见3种杂菌消毒试验  

Disinfection Test on Three Kinds of Bacteria in Edible Fungi Plant Treated with Sodium Percarbonate and Microwave

作　　者：张繁荣[1] 胡志辉[1] 雷刚[1] 周世力[1] 孙齐英[1] 

机构地区：[1]江汉大学生命科学学院,湖北武汉430056

出　　处：《江汉大学学报（自然科学版）》2014年第4期69-72,共4页Journal of Jianghan University：Natural Science Edition

基　　金：武汉市科学计划项目(201220837304-2)

摘　　要：试验选用在食用菌培养基中最常见的、不易被杀死的3种菌(大肠埃希菌、枯草芽孢杆菌、黑曲霉菌)作为消毒灭菌试验对象,对过碳酸钠和微波的消毒灭菌效果进行测试。过碳酸钠采用20 000、10 000、1 000、100、50、10mg/L 6种浓度梯度进行试验。试验表明随着过碳酸钠浓度升高3种菌的数量均在减少。大肠埃希菌与黑曲霉菌在浓度为10 000 mg/L时全部被杀死,枯草芽孢杆菌在浓度为20 000 mg/L时全部被杀死。随着微波时间的增加菌的数量逐渐减少,在微波时间为210 s时黑曲霉菌全部被杀死,240 s时大肠埃希菌全部被杀死,300 s时枯草芽孢杆菌全部被杀死,达到了彻底灭菌的效果。Selected three kinds of bacteria which are most common in mushroom culture medium and are difficult to be killed（E. coli,Bacillus subtilis,Aspergillus）as the test object,to test the steril-ization effect of sodium percarbonate and microwave. The sodium percarbonate had the concentration gradient as 20 000,10 000,1 000,100,50,10 mg/L. The experiments showed the amount of three bacteria reduced as the concentration of sodium percarbonate increased. The E. coli and the Aspergil-lus were all killed at the concentration of 10 000 mg/L,the Bacillus subtilis were all killed at the concentration of 20 000 mg/L. As the time of microwave increased,the amount of bacteria reduced, the Aspergillus were all killed at 210 s of microwave,the E. coli were all killed at 240 s of micro-wave,the Bacillus subtilis were all killed at 300 s of microwave,the experiment reached the com-plete sterilization.

关 键 词：过碳酸钠 微波 消毒 灭菌 食用菌培养基 

分 类 号：S482.2[农业科学—农药学] S646[农业科学—植物保护]