利用新型生物聚酯P3HB4HB构建原位催化NO生成人工血管材料  

CONSTRUCTION OF VASCULAR GRAFT MATERIAL WITH in situ CATALYZED NITRIC OXIDE GENERATION USING P3HB4HB

作　　者：李彦[1] 陈思原[1] 张雅薇[1] 张丽妍[1] 王淑芳[1] 

机构地区：[1]南开大学生命科学学院,生物活性材料教育部重点实验室,天津300071

出　　处：《离子交换与吸附》2014年第4期289-296,共8页Ion Exchange and Adsorption

基　　金：国家"973"计划(2012CB725204);国家自然科学基金(51073081;J1103503);天津市自然科学基金(13JCYBJC24900)

摘　　要：以新型生物聚酯P3HB4HB静电纺丝纤维支架材料为基质,通过层-层自组装技术向基质中引入γ-聚谷氨酸和聚赖氨酸,并负载含硒催化剂硒代胱胺,构建具有催化NO原位生成功能的人工血管材料,通过体外催化释放NO实验、细胞毒性检测、平滑肌细胞铺展实验考察人工血管材料的生物功能。实验结果表明,该材料具有良好的纤维多孔结构,适于作血管支架材料;通过控制有机硒的负载量可调控NO催化生成速率;而且该材料具有良好的生物相容性,能够有效抑制平滑肌细胞的黏附与铺展。Small-diameter vascular grafts are increasingly needed in the clinic. In this paper, a novel vascular graft was constructed in situ catalytic nitric oxide generation. Poly（？,-glutamic acid） and poly（a-lysine） were alternatively deposited onto the surface of electrospun P3HB4HB matrix via electrostatic layer-by-layer self-assembly, and then selenocystamine was loaded as a catalyst. The biological functions of the vascular grafts were tested via determination of nitric oxide generation, cellular proliferation experiment and spreading of smooth muscle cell experiment. As a result, the fibrous structure with pores of electrospun P3HB4HB was suitable as scaffold of vascular grafts. The results of determination of in vitro catalytic nitric oxide generation demonstrated that this catalyst-loaded material could significantly accelerate the release of nitric oxide from S-nitrosoglutathione. The material possessed good cellular compatibility. Besides, the catalyst-loaded material exhibited the capability of inhibiting smooth muscle cell spreading.

关 键 词：人工血管 层-层自组装 静电纺丝 P3HB4HB 硒代胱胺 催化生成 

分 类 号：TQ342.87[化学工程—化纤工业]