丙型肝炎病毒对白细胞介素32表达影响的研究  

Study on the effect of Hepatitis C virus(HCV) on the expression of interleukin 32

作　　者：祝成亮[1] 李艳[1] 刘兴晖[2,3] 叶芳丽[1] 

机构地区：[1]武汉大学人民医院检验科,湖北武汉430060 [2]上海市浦东新区公利医院检验科,上海200135 [3]湖北医药学院附属东风医院检验科,湖北十堰442008

出　　处：《海南医学》2014年第18期2708-2709,共2页Hainan Medical Journal

基　　金：国家临床重点专科专项经费(编号:2010305);国家自然科学基金(编号:81101485);湖北省教育厅科学技术研究项目(编号:D20122404)

摘　　要：目的探讨丙型肝炎病毒(HCV)在体内外对白细胞介素32(IL-32)表达的影响。方法荧光定量PCR和免疫印迹法检测Huh7.5.1细胞中IL-32 mRNA和蛋白的表达水平,酶联免疫吸附试验(ELISA)检测IL-32的血清含量。结果 HCV感染的Huh7.5.1细胞IL-32 mRNA和蛋白的表达水平较对照细胞高,HCV患者IL-32血清含量明显高于健康对照(P<0.05)。结论 HCV能够在体内外促进IL-32的合成和分泌。Objective To explore the effect of hepatitis C virus （HCV） on the expression of interleukin 32 （IL-32）. Methods The mRNA and protein expression of IL-32 in Huh7.5.1 cells were measured by real-time PCR and western blot. Serum levels of IL-32 were measured by enzyme-linked immunosorbent assay （ELISA）. Results Ex-pression of IL-32 mRNA and protein was higher in Huh7.5.1 cells infected by HCV than in the control cells. Serum IL-32 levels was significantly higher in HCV patients as compared with healthy controls （P〈0.05）. Conclusion HCV can upregulate the synthesis and secretion of IL-32 both in vivo and in vitro.

关 键 词：丙型肝炎病毒 白细胞介素32 酶联免疫吸附试验 表达 

分 类 号：R512.63[医药卫生—内科学]