老年期痴呆模型大鼠炎性因子变化相关性研究  

Correlative Research about Change in Inflammatory Cytokines of Senile Dementia Rat Model

作　　者：韩诚[1] 郭蕾[1] 陈燕清[1] 秦亚莉[2] 于红[3] 郭斌[1] 

机构地区：[1]山西中医学院,山西太原030024 [2]山东中医药大学基础医学院,山东济南250355 [3]山西省中医药研究院,山西太原030012

出　　处：《辽宁中医杂志》2014年第9期1990-1993,共4页Liaoning Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金(81173165;81202623)

摘　　要：目的:观察老年期痴呆病证结合模型大鼠白细胞介素-6(IL-6)和肿瘤坏死因子-α(TNF-α)含量,剖析IL-6及TNF-α含量的变化是否能成为反映两种证型的特异性微观指标。方法:采用间隔一周分别结扎左、右颈总动脉造成痴呆模型,叠加腹腔注射D-半乳糖建立老年期痴呆大鼠模型,在疾病模型基础上分别施以投喂高脂饲料和低温环境刺激,建立两种病证结合(痰浊阻窍证和寒凝血瘀证)的老年期痴呆大鼠模型。应用Morris水迷宫实验检测大鼠学习记忆能力,测定IL-6和TNF-α的含量。结果:老年期痴呆组、痰浊阻窍证组、寒凝血瘀证组大鼠的IL-6及TNF-α含量显著高于正常组(P<0.05),老年期痴呆组、痰浊阻窍证组、寒凝血瘀证组大鼠之间则无显著性差异(P>0.05)。结论:IL-6、TNF-α不适合作为寒凝血瘀证、痰浊阻窍证的特异性生物标记物。Objective ： To investigate the interleukin 6 （ IL - 6 ） and tumor necrosis factor - alpha（ TNF -α ） levels of the senile dementia rat model that combines relational diseases and syndromes and to analyze whether the change in IL - 6 and TNF -α levels could become a reflection of two syndromes specific microeconomic indicators. Methods ： By ligating the left and right carotid arteries one week apart, the VD model was established, superimposing by intraperitoneal injection of D - galaetose to build senile dementia rat model. The model rats were given diet food and exert low temperature environmental, respectively creating two rat models of senile dementia rat model that combined relational diseases and syndromes （ the syndrome of phlegm and turbid sub- stance blocking orifices and the syndrome of cold coagulation and blood stasis） . We tested the learning and memory abilities of rats by Morris water maze and determined the content levels of IL - 6 and TNF -α. Results ： The senile dementia group, the phlegm and turbid substance blocking orifices syndrome group and the cold coagulation and blood stasis group rats＇ TNF -α and IL -6 levels were significantly higher than those of the normal group（ P 〈 0.05 ） and there was no significant difference among the senile dementia group, the phlegm and turbid substance blocking orifices syndrome group and the cold coagulation and blood stasis group （ P 〉 O. 05 ）. Conclusion： IL - 6 and TNF -α are not the suitable specificity biotic signs for the syndrome of phlegm and turbid substance blocking orifices and the syndrome of cold coagulation and blood stasis.

关 键 词：老年期痴呆 病证结合 寒凝血瘀证 痰浊阻窍证 炎性因子 

分 类 号：R285[医药卫生—中药学]