益气化瘀解毒方及拆方对人肝癌HepG2裸鼠移植瘤和HIF1α、E-cad表达的影响  

Effect of Yiqi Huayu Jiedu Formula and Its Separates on Nude Mice Transplantation Tumor of HepG2 Cells and Expression of HIF1a and E-cad

作　　者：曾普华[1] 郜文辉[2] 潘敏求[1] 蒋益兰[1] 朱克俭[1] 李勇敏[1] 李跃辉[1] 唐珍[1] 

机构地区：[1]国家中医药管理局重点学科,湖南省中医药研究院附属医院肿瘤科,湖南长沙410006 [2]湖南中医药大学中医学院,湖南长沙410208

出　　处：《辽宁中医杂志》2014年第9期2004-2006,共3页Liaoning Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(81001522);湖南省自然科学基金课题(13JJ4138);首届中国中西医结合学会-金港榄香烯中医药肿瘤研究基金资助(2010-F10)

摘　　要：目的:观察益气化瘀解毒方对人肝癌移植瘤及HIF1a、E-cad表达的影响。方法:建立人肝癌移植瘤模型,分为7组,分别以益气化瘀解毒方、黄芪、莪术、重楼、壁虎、顺铂药物干预21 d后,取瘤组织,行免疫组化检测。结果:(1)成瘤体积比较:给药后瘤体积及差值比较,各组与空白组均有显著性差异(P<0.05);全方组分别与黄芪组、重楼组、壁虎组、空白对照组等比较有显著性差异(P<0.05)。(2)对HIF1a的影响:全方组与黄芪组比较无显著性差异(P>0.05),与其它组比较有显著性差异(P<0.05);各组与空白组比较有显著性差异(P<0.05);莪术组、壁虎组、顺铂组间比较无显著性差异(P>0.05)。(3)对E-cad影响:全方组与黄芪组比较无显著性差异(P>0.05),莪术、重楼、壁虎组与空白组比较无显著性差异(P>0.05)。结论:(1)益气化瘀解毒方全方较单味中药具有较强的抑瘤作用,其组方中药如莪术、重楼、壁虎均有一定程度抑瘤作用,莪术抑瘤作用较强;组方内主要单味中药发挥了协同抑瘤效应,使整方呈现出最强的抑瘤效应。(2)益气化瘀解毒方能抑制HIF1a表达,促进E-cad表达,可能通过阻断上皮间质转化而发挥抗肝癌转移侵袭作用,组方中药对其作用各有侧重,但作为整方的有机组成从不同环节发挥了协同作用。Objective：To observe the effect of Yiqi Huayu Jiedu （YQHYJD） formula and its separate on nude mice transplanta- tion tumor of hepatoma cells and the expression of HIF1 a and E-cad. Methods ： Models of nude mice transplantation tumor are di- vided into 7 groups, treated by YQHYJD, astragalus, rhizoma zedoariae, paris polyphylla, gecko and cisplatin, after 21 days, immu- nohistochemical detection in tumor tissue. Results ： （ 1 ） The effect on tumor volume ： There was significant difference between the black and the other except Astragalus （ P 〉 0. 05 ）. There was significant difference between cisplatin and the other（ P 〈 0. 05 ）. There was significant difference between YQHYJD and the separates （P 〈 0. 05 ） . （2） The effect on HIF1 a：There was significant difference between the black and the other（ P 〈 0. 05 ）. There was significant difference between YQHYJD and the other drugs ex- cept Astragalus （ P 〈 0. 05 ）. （ 3 ） The effect on E-cad ： There was significant difference between YQHYJD and the other drugs ex- cept Astragalus（ P 〈 0. 05 ）. There was no significant difference between rhizoma zedoariae, paris polyphylIa, gecko and the black （ P 〉 0. 05）. Conclusion ： Yiqi Huayu Jiedu formula plays a better effect of anti-tumor than Single herbs, rhizoma zedoariae, paris polyphylla, and gecko has the effect of anti-tumor, Yiqi Huayu Jiedu formula can inhibit the expression of HIF1 a and E-cad. rhizoma zedoariae, paris polyphylla, and gecko has on effect on E-cad.

关 键 词：益气化瘀解毒方 人肝癌HePG2裸鼠移植瘤 HIF1a E-CAD 

分 类 号：R285[医药卫生—中药学]