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作 者:侯永革[1] 王保芝[2] 王金立[2] 王俊峰[2] 崔芳[3] 孟丽[3]
机构地区:[1]河北省石家庄市第一医院神经内科,河北石家庄050011 [2]河北医科大学基础医学院人体解剖学教研室,河北石家庄050017 [3]河北医科大学基础医学研究所电镜实验中心,河北石家庄050017
出 处:《河北医科大学学报》2014年第9期997-999,F0002,共4页Journal of Hebei Medical University
基 金:河北省卫生厅重点科研项目(20120044)
摘 要:目的探讨大鼠侧脑室脉络丛及丛上细胞的结构特点。方法选用成年雄性SD大鼠5只,在深度麻醉下通过主动脉灌注醛类固定液,取侧脑室脉络丛组织制备样品,进行扫描电镜和透射电镜观察。结果扫描电镜观察显示,侧脑室脉络丛表面由平行方式形成的皱襞或脊组成,上皮细胞游离面可见大量微绒毛;脉络丛表面可见许多丛上细胞,其胞体带有伪足样突起。透射电镜观察显示,脉络丛上皮细胞下为有孔型毛细血管,上皮细胞之间为缝隙连接;脉络丛上皮细胞内有丰富的线粒体和分泌囊泡;丛上细胞的细胞体和伪足状突起内,可见次级溶酶体或吞噬体。结论大鼠侧脑室脉络丛及丛上细胞的细微和超微结构特点,为深入研究其生理和病理意义,提供了形态学基础。OcjectiVe The study was to eXamine structural features of the choroid pleXus and epipleXus cells of the rat lateral ventricles. Methods Five adult male SD rats were anesthetized deeply with 6% chloral hydrate intra-peritoneal cavity and aortic perfusion with 3% polyformaldehyde and 1%glutaraldehyde,the samples of the choroid pleXus were collected and observed with scanning electron microscope( SEM)and transmission electron microscope( TEM). Results SEM showed the surface of the choroid pleXus of the rat lateral ventricles was constituted by folds or crests in a kind of parallel way,the free surface of the cells presented microvilli. There were pseudopodial projections on the surface of epipleXus cells. TEM showed that fenestrated capillaries can be seen in choroid pleXus subepithelial cells, gap junctions can be observed between epithelial cells,a lot of mitochondria and endocytosis bubbles can be seen in cytoplasm of epithelial cells. There were pseudopodial projections on the surface of epipleXus cells. Secondary lysosome can be seen in epipleXus cells. Conclusion The study showed that the fine and ultra-structural features of the choroid pleXus and epipleXus cells of the rat lateral ventricles may provide a morphological basis for further research of the physiological and pathological significance.
分 类 号:R322.811[医药卫生—人体解剖和组织胚胎学]
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