杆状病毒表面展示系统的发展及应用  

Development and application of Baculovirus surface display system

作　　者：郑浩[1] 徐亮亮[1] 韦磊[1] 孙京臣[1] 

机构地区：[1]华南农业大学动物科学学院,广东广州510642

出　　处：《广东蚕业》2014年第2期29-33,共5页Guangdong Sericulture

基　　金：国家自然科学基金(资助号31372373)

摘　　要：杆状病毒表面展示系统(Baculovirus Surface Display,BSD)是近年来发展起来的一种崭新的膜展示技术。其主要原理是通过基因工程的方法,将杆状病毒表面囊膜蛋白基因与所要展示的目的蛋白基因进行融合,形成重组杆状病毒。重组杆状病毒的囊膜蛋白在表达同时,目的蛋白也随即进行表达,并与囊膜蛋白共同运输至病毒囊膜处,特异的与锚定部位结合,展示在杆状病毒表面。该系统由于具有较强的可控性和较高的展示效率,目前已经被广泛应用于基因转导与基因治疗、较复杂的真核蛋白展示、新型疫苗研发以及单克隆抗体的制备等领域。本文对杆状病毒表面展示系统的研究现状进行了阐述和总结,并对其发展和应用前景做出了展望。Successful examples in Baculovirus Surface Display system （BSD） have recently been achieved by the recombinant DNA technique via recombinant DNA technology. The main principle is to combine the baculovirus surface envelope protein genes and the target genes and to express a fusion protein via the recombinant baculovirus. The envelope protein of recombinant baculovirus is expressed, meanwhile, the target protein is expressed as well. The target genes are expressed and transported to virus envelope with the envelope protein, then specifically anchored and displayed on the baculovirus surface. Due to the strong controllability and high efficiency, BSD has been widely used in gene transduction, gene therapy, complex eukaryotic proteins display, new vaccine development and the preparation of monoclonal antibodies, etc. In this paper, the research status quo of baculovirus surface display system is reviewed, including the development and application prospect .

关 键 词：杆状病毒 表面展示 囊膜蛋白 融合表达 

分 类 号：Q786[生物学—分子生物学]