舞毒蛾LdOA1基因克隆分析及对3种杀虫剂胁迫的响应  

Cloning and Analysis of LdOA1 in Lymantria dispar and Its Response to the Stress of Three Kinds of Insecticides

作　　者：曹传旺[1] 孙丽丽[1] 问荣荣 豆晓洁[1] 王志英[1] 

机构地区：[1]东北林业大学林学院,哈尔滨150040

出　　处：《林业科学》2014年第8期102-107,共6页Scientia Silvae Sinicae

基　　金：国家自然科学基金项目(31101676);东北林业大学青年拔尖人才支持计划(PYTT-1213-10);中央高校基本科研业务费专项基金(DL11BA02)

摘　　要：G蛋白偶联受体(GPCRs)是生物体内重要膜受体,通过激活三聚体G蛋白参与各种细胞内信号转导,在药物研发中具有重要的作用。从舞毒蛾无参照全转录本文库中鉴定获得GPCR家族中眼白化I型全长基因(命名为LdOA1),该基因全长453 bp,编码150个氨基酸,分子质量为17.54 kDa,理论等电点(pI)为9.76。进化树分析表明LdOA1蛋白与帝王蝶的亲缘关系较近。实时荧光定量PCR分析表明舞毒蛾3龄幼虫在溴氰菊酯、甲萘威和氧化乐果胁迫下LdOA1的表达均表现为显著抑制。The G protein coupled receptors （ GPCRs） ,known as transmembrane receptors,play an important role in drug research and development by activating G protein involved in inside signal transduction pathways. In present study, the full length cDNA of GPCR （namely LdOA1） was isolated from Lymantria dispar transcriptome. The open reading frame （ ORF） of LdOA1 was 453 bp encoding a protein of 150 amino acid residues with the molecular mass of 17. 54 kDa and theoretical pI of 9. 76. Phylogenetic analysis of GPCR proteins showed LdOA1 of L. dispar clustered into a group with Danaus plexippus. We further investigated the expression of LdOA1 in 3rd instar L. dispar larvae under deltamethrin, carbaryl and omethoate stresses using real-time PCR. The results showed that LdOA1 in L. dispar was obviously inhibited by deltamethrin,carbaryl and omethoate.

关 键 词：舞毒蛾 LdOA1 基因 杀虫剂 基因表达 

分 类 号：S718.7[农业科学—林学]