云南灯盏花胶囊对慢性肾衰竭大鼠CTGF及Smad 7mRNA表达影响的研究  

Yunnan Erigeron breviscapus capsules on expression of CTGF and Smad7 mRNA in rats with chronic renal failure: an experimental study

作　　者：杜义斌[1] 李琦[1] 张坤扬 段艳蕊[1] 吴晓[1] 谢楚侨[1] 刘明星[1] 潘承政 

机构地区：[1]云南中医学院临床医学院,昆明650021

出　　处：《环球中医药》2014年第9期666-669,共4页Global Traditional Chinese Medicine

基　　金：云南省自然科学基金面上项目(2010ZC194)

摘　　要：目的通过观察云南灯盏花胶囊对5/6肾切除慢性肾衰竭大鼠结缔组织生长因子(connective tissue growth factor,CTGF)、Smad7蛋白表达的影响,探讨云南灯盏花胶囊减轻肾纤维化以延缓慢性肾衰竭的作用机制。方法采用5/6肾切除法造模,观察实验大鼠治疗后的一般状态。检测血肌酐(serum creatinine,Scr)、血尿素氮(blood urea nitrogen,BUN)摘取残肾组织,RT-PCR检测肾组织CTGF的表达,蛋白质印迹技术(Western Blot)法检测肾组织内Smad7 mRNA的表达。结果云南灯盏花胶囊组慢性肾衰竭(chronic renal failure,CRF)大鼠BUN、Scr下降,肾组织CTGF表达减弱,Smad7 mRNA表达增强,与模型组比较差异有统计学意义(P<0.05)。结论云南灯盏花胶囊可明显改善5/6肾切除CRF大鼠肾功能,升高Smad7 mRNA表达,减少肾纤维化因子CTGF表达,延缓慢性肾衰竭进展。Objective By observing the Yunnan Breviscapine capsules on 5/6 nephrectomy chron- ic renal failure rats the influence of connective tissue growth factor（CTGF） , Smad7 protein expression, dis- cuss the mechanism of Yunnan Breviscapine capsules on relieving renal fibrosis to delay the chronic renal failure. Methods 5/6 renal resection method is adopted to build mould, observe the experimental rats＇ general status after treatment. Serum creatinine, and blood urea nitrogen are detected, and residual kidney tissues are picked, RT-PCR is used to deteet renal tissue CTGF expression, and Protein imprinting technol- ogy（Western Blot） is used to detect renal tissues Smad7 mRNA expression. Results The BUN and Scr Decreased in Yunnan Breviscapine capsules group of chronic renal failure rats. CTGF express abate of Kid- ney tissue, Smad7 mRNA Express to enhance of Kidney tissue, Compared with model group, difference was statistically significant（P 〈 0. 05）. Conclusion Yunnan Breviscapine capsules can obviously improve the 5/6 nephrectomy CRF rats＇ renal function, elevate Smad7 mRNA expression, reduce renal fibrosis factor CTGF expression, and delay chronic renal failure.

关 键 词：云南灯盏花胶囊 慢性肾衰竭 结缔组织生长因子 实验研究 

分 类 号：R285.5[医药卫生—中药学]