基于金黄膏体外透皮吸收实验探讨其透皮系统的药对配伍规律  

Investigation on the compatibility law of in vitro transdermal absorption of transdermal system drugs based on Jinhuang Cream experiment

作　　者：曹颖[1] 王红[2] 刘俊红[3] 韩俊泉[2] 刘斌[2] 李棣华[3] 吕沅姗 

机构地区：[1]天津中医药大学研究生院 [2]天津中医药大学第二附属医院外一科 [3]天津市中西医结合急腹症研究所药物研究室

出　　处：《环球中医药》2014年第9期691-694,共4页Global Traditional Chinese Medicine

基　　金：国家自然科学基金(81072818)

摘　　要：目的通过进行金黄膏中小檗碱体外透皮吸收实验,探讨金黄膏中不同药对配伍[1]对其透皮效果的影响,进一步探讨其影响机制。方法采用体外经皮渗透法,使用透皮扩散试验仪,选择大鼠腹部皮肤为渗透屏障,以30%乙醇—磷酸盐缓冲溶液(pH=6.8)作为接受液,用高效液相色谱法测定接受液中小檗碱含量。考察全方药组及缺姜黄、白芷组(缺药方A组)和缺姜黄、白芷、天南星、陈皮、厚朴组(缺药方B组)中小檗碱的透皮特性。结果 (1)缺药方A组和缺药方B组的稳态透皮速率、透皮时滞、32小时内小檗碱单位面积累积透过量都较全方高。(2)金黄散全方组的稳态透皮速率及32小时内盐酸小檗碱累积透过量都低于缺姜黄、白芷的缺药方A组,说明姜黄、白芷对小檗碱的皮肤渗透有抑制作用。(3)有天南星、陈皮、厚朴的缺药方A组的小檗碱透过量高,而透皮时滞低于缺药方B组,说明天南星、陈皮、厚朴对小檗碱的皮肤渗透有促进作用。结论金黄膏中的姜黄、白芷和天南星、陈皮、厚朴这两组药都对金黄膏发挥疗效起到了促进作用。金黄膏中每组药对都各负其责,又相互协同,合理配伍组成一个整体的透皮系统。Objective By performing berberine in vitro percutaneous absorption experiments to ex-plore the transdermal effect of the different compatibility of drugs in the Jinhuang Cream [1] and then to fur-ther explore the influencing mechanism .Methods In this study, in vitro percutaneous penetration method was used and transdermal diffusion test instrument was applied .Rat abdominal skin was selected as perme-ability barrier and 30% ethanol-phosphate buffer solution （ pH=6.8） was chosen as the accepted solu-tion..The concentration of berberine in the accepted solution was measured by HPLC method to examine the transdermal properties of the whole prescription , the lack of Turmeric , Angelica dahurica group （ lack of prescription group A） and the lack of Turmeric, Angelica dahurica, Araceae, Citrus, Magnolia group （ lack of prescription group B ） drugs.Results （1） Compared with the whole prescription group , the in-dexes of steady-state permeation rate, transdermal delay, 32h accumulated berberine unit area by volume and permeability were much highert in Lack of prescription group A and lack of prescription group B .（2） The steady state permeation rate and 32h accumulated berberine unit area by volume and permeability in the whole prescription group were both lower than that in the the lack prescription group A , which demon-strated that, Turmeric, Angelica dahurica can restrain the skin penetration of berberine .（3） The steady-state permeation rate in lack of prescription group A was higher than in lack of prescription group B , while and transdermal delay in lack prescription group A was lower than that in lack prescription group B .The results suggested that Araceae , Citrus, Magnolia can promote skin penetration of berberine .Conclusion Turmeric, Angelica dahurica and Araceae , Citrus, Magnolia both sets of the drugs in Jinhuang Cream can effective promote the percutaneous absorption of golden cream .Each drug in Jinhuang Cream plays an im-portant role on the compatibility , mutual collaboratio

关 键 词：金黄膏 小檗碱 透皮吸收 透皮系统 

分 类 号：R944.21[医药卫生—药剂学]