灵芝孢子油的稳定性考察  

Stability of Ganoderma Spores Oil

作　　者：曾荣华[1] 李菁[1] 刘翠红[1] 宋晓燕[1] 

机构地区：[1]广州白云山汉方现代药业有限公司中药提取分离过程现代化国家工程研究中心,广州510240

出　　处：《中国实验方剂学杂志》2014年第18期23-25,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家"重大新药创制"科技重大专项(2011ZX09401-404);广州市科技计划项目(201300000084)

摘　　要：目的：考察灵芝孢子油加速试验后的质量变化.方法：以酸值、过氧化值、总三萜及甘油三油酸酯含量为评价指标,考察灵芝孢子油加速0,1,2,3,6个月后的质量变化.采用HPLC测定甘油三油酸酯含量,Kromasil-C18色谱柱（4.6 mm×200 mm,5μm）,流动相乙腈-异丙醇（53∶47）,流速0.5 mL·min-1,蒸发光散射检测器检测,柱温30℃.结果：与0月相比,灵芝孢子油加速6个月后,酸值呈上升趋势,上升幅度32.11％ ～39.27％;过氧化值呈下降趋势,下降幅度41.17％ ～ 46.67％;总三萜含量呈下降趋势,下降幅度6.72％～8.13％;甘油三油酸酯含量呈下降趋势,下降幅度3.78％～4.54％;酸值与过氧化值均符合食用植物油的卫生标准.结论：灵芝孢子油加速6个月质量较稳定.Objective：To investigate stability of Ganoderma spores oil experienced by six months accelerated test.Method：Taking acid value,peroxide value,contents of total triterpenoids and glycerol trioleate as indexes,quality changes of spores oil after 0,1,2,3,6 months accelerated test were investigated.The content of glycerol trioleate was determined by HPLC,which was performed on a Kromasil C18 analytical column with mobile phase of acetonitrile-isopropanol （53 ∶ 47） at flow rate of 0.5 mL ·min-1,column temperature was 30 ℃,detector was evaporative light-scattering.Result：Compared with zero month,after 6 months accelerated test of Ganoderma spores oil,acid value showed a rising trend with rising 32.11％-39.27％; peroxide value showed a decreasing trend with decreasing 41.17％-46.67％ ; the content of total triterpenoids showed a decreasing trend with the range of 6.72％-8.13％ ; the content of glycerol trioleate showed a decreasing trend with dropping by 3.78％-4.54％ ; acid and peroxide values were accorded with gienic standard for edible vegetable oil during 6 months accelerated test.Conclusion：Quality of Ganoderma spores oil remains stable after 6 months accelerated test.

关 键 词：灵芝孢子油 稳定性 加速试验 甘油三油酸酯 总三萜 

分 类 号：R283.6[医药卫生—中药学] R284.1[医药卫生—中医学]