湿毒清胶囊中地黄的提取工艺优化及其切制前后药效对比  

Optimization of Extraction Technology for Rehmanniae Radix from Shiduqing Capsules and Comparison of Its Pharmacodynamic Action Before and After Being Processed

作　　者：覃柳珍[1] 覃葆[2] 刘泉[2] 林威[2] 韦东晓[2] 仝丹丹[2] 贺勤[2] 梁悦[2] 

机构地区：[1]广西医科大学第四附属医院,广西柳州545005 [2]广西中医药大学,南宁530001

出　　处：《中国实验方剂学杂志》2014年第18期38-41,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家科技特派员项目(2009GJE10028)

摘　　要：目的:优选湿毒清胶囊中地黄的提取工艺并比较地黄切制前后的药效。方法:采用HPLC测定梓醇含量,流动相乙腈-0.1%磷酸溶液(1∶99),检测波长210 nm。以梓醇提取量为指标,采用正交试验考察粉碎度、料液比、提取时间对地黄提取工艺的影响。以生理盐水为空白对照,地塞米松为阳性对照,通过抗炎镇痛试验比较地黄药材切制前后高(16 g·kg-1)、低剂量组(8 g·kg-1)的药效差异。结果:最佳提取工艺为粉碎成5 mm小块,加20倍量水提取2次,每次1.5 h。切制前后高剂量组对二甲苯诱发小鼠耳廓肿胀的抑制率分别为33.72%,48.34%;低剂量组依次为43.13%,64.31%。结论:该工艺稳定可行,为湿毒清胶囊的工业生产提供参考。Objective：To optimize extracting technology of Rehmanniae Radix in Shiduqing capsules and compared its pharmacodynamic action before and after being processed.Method：HPLC was employed to determine the content of catalpol with mobile phase of acetonitrile-0.1％ phosphoric acid （1 ∶ 99） and detection wavelength at 210 nm.With extracting amount of catalpol as index,orthogonal test was adopted to optimize extraction technology by taking liquid-solid ratio,grinding degree and extracting time as factors.With physiological saline as blank control and dexamethasone as a positive control,pharmacodynamic action of Rehmanniae Radix before and after being processed was compared by anti-inflammatory and analgesic test.Result：Optimum extracting technology was as following：shattered into 5 mm small pieces and extracted twice with 20 times the amount of water for 1.5 hours per time.Before and after being cut,inhibition rates for xylene-induced auricle swelling in mice of high-dose （16 g ·kg-1） groups were 33.72％ and 48.34％,while low-dose groups were 43.13％ and 64.31％,respectively.Conclusion：This optimized extracting technology is stable and feasible,which provides a reference for industrial production of Shiduqing capsules.

关 键 词：湿毒清胶囊 地黄 梓醇 抗炎镇痛试验 

分 类 号：R283.6[医药卫生—中药学] R285.5[医药卫生—中医学]