UPLC测定银黄颗粒中绿原酸、咖啡酸、黄芩苷、汉黄芩苷、黄芩素和汉黄芩素6种成分  

Simultaneous Determination of Six Flavones in Yinhuang Granule by UPLC

作　　者：张建伟[1,2] 赵倩[3,4] 何希荣[3] 冯伟红[3,4] 

机构地区：[1]河南中医学院第二附属医院 [2]河南省中医院肾内科,郑州450002 [3]中国中医科学院中药研究所,北京100700 [4]中药质量控制技术国家工程实验室,北京100700

出　　处：《中国实验方剂学杂志》2014年第18期50-53,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家“重大新药创制”科技重大专项(2009ZX09301-005,2009ZX09308-003)

摘　　要：目的:建立采用UPLC同时测定银黄颗粒中绿原酸、咖啡酸、黄芩苷、汉黄芩苷、黄芩素和汉黄芩素6种成分含量的方法。方法:采用ACQUITY UPLC BEH C18色谱柱(2.1 mm×50 mm,1.7μm),流动相乙腈-0.2%磷酸水梯度洗脱,在326nm波长处进行检测。结果:绿原酸、咖啡酸、黄芩苷、汉黄芩苷、黄芩素和汉黄芩素的线性关系良好,加样回收率分别为96.34%(RSD 0.90%),101.4%(RSD 1.53%),95.64%(RSD 1.34%),98.01%(RSD1.72%),98.61%(RSD 1.96%),102.7%(RSD 1.42%)。结论:该方法简便、快速,分离效果好,可为全面控制银黄颗粒的质量提供参考。Objective：This study was aimed to develop an UPLC method to determine the contents of chlorogenic acid,caffeic acid,baicalin,wogonoside,baicalein and wogonin in Yinhuang granule.Method：Six active components were separated on an ACQUITY UPLC BEH C18column （2.1 mm ×50 mm,1.7 μm） eluted with acetonitrile and water containing 0.2％ phosphoric acid as mobile phases in a linear gradient mode.The detection wavelength was set at 326 nm.Result：The linear ranges were 0.000 107-0.107 μg for chlorogenic acid,0.000 244-0.244 μg for caffeic acid,0.000 272-0.272 μg for baicalin,0.000 059 0-0.059 0 μg for wogonoside,0.000 053 0-0.053 μg for baicalein,0.000 051 0-0.051 μg for wogonin,respectively.The average recoveries of the six components were 96.34％ （RSD 0.90％）,101.4％ （RSD 1.53％）,95.64％ （RSD 1.34％）,98.01％ （RSD1.72％）,98.61％ （RSD 1.96％）,102.7％ （RSD 1.42％）,respectively.Conclusion：The method was proven to be simple,accurate,reliable and can be used for quality control of the six flavones inYinhuang granule.

关 键 词：超高液相色谱 银黄颗粒 绿原酸 咖啡酸 黄芩苷 汉黄芩苷 黄芩素 汉黄芩素 

分 类 号：R284.1[医药卫生—中药学]