同株凤丹皮的不同部位中丹皮酚和芍药苷的含量比较  

Content Comparison of the Paeonol and Paeoniflorin on Different Part of One Feng-cortex Moutan

作　　者：纪开明 方成武[2,3] 管玉云 管大平 吴计划 

机构地区：[1]铜陵市食品药品检验中心,安徽铜陵244000 [2]安徽中医药大学,合肥230031 [3]亳州职业技术学院,安徽亳州236800 [4]北京同仁堂安徽中药材有限公司,安徽铜陵244000

出　　处：《中国实验方剂学杂志》2014年第18期54-56,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家“十二五”科技支撑计划(2011BAI04B05);国家中医药管理局项目(201107009);安徽省食品药品监督管理系统科研资助项目(0011)

摘　　要：目的：采用高效液相色谱法,比较同地同株凤丹皮中不同部位的丹皮酚和芍药苷含量差异.方法：采用WatersVP-ODS-C18色谱柱（4.6 mm×150 mm,5μm）;丹皮酚含量测定流动相甲醇-水（55∶45）,检测波长274 nm,柱温35℃,流速1.0mL·min-1;芍药苷含量测定流动相乙腈-0.1％H3PO4（20∶ 80）,检测波长230 nm,柱温35℃,流速1.0 mL· min-1.结果：丹皮酚在1.073 ～5.365 μg,芍药苷在0.593 ～2.965μg均呈良好线性关系,相关系数分别为0.999 9,0.999 1,平均回收率丹皮酚为99.96％（RSD 0.72％）,芍药苷为99.03％（RSD 0.49％）.结论：在选定的同株凤丹皮最细部位的丹皮酚和芍药苷含量均高于最粗部位及中等部位.Objective：Analysis and comparison the paeonol and paeoniflorinon different part of one Feng-Cortex Moutan by HPLC.Method：Using the Waters VP-ODS-C18chromatographic column （4.6 mm × 150mm,5 μm）.Mobile phase for the paeonol：methanol-water （55∶45）,detection wavelength 274 nm,column temperature 30 ℃,the flow rate of 1.0 mL ·min-1.Mobile phase for thepaeoniflorin：acetonitrile-0.1％ H3PO4 （20∶80）,detection wavelength 230 nm,column temperature 30 ℃,the flow rate of 1.0 mL ·min-1 Result：Paeonol and paeoniflorin had good linear relationship in 1.073-5.365 μg and 0.593-2.965 μg,the ‘ r’ was 0.999 9 and 0.999 1,the average recoveriey for the paeonol was 99.96％ （RSD 0.72％）,the average recovery for the paeoniflorin was 99.03％ （RSD 0.49％）.Conclusion：The experiments show that the content of the paeonol and paeoniflorin localed at the thinest part of the chosen Feng-Cortex Moutan is much more higher than the rest part.

关 键 词：凤丹皮 丹皮酚 芍药苷 不同部位 

分 类 号：R284.1[医药卫生—中药学]