木蝴蝶提取物对酪氨酸酶的抑制及抗氧化作用  

Effect on Anti-tyrosinase and Antioxidation Activities of Extract from Oroxylum indicum

作　　者：王锐[1] 熊汝琴[1] 罗家刚[1] 祁岑[1] 

机构地区：[1]昭通学院化学与生命科学学院,云南昭通657000

出　　处：《中国实验方剂学杂志》2014年第18期70-72,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：云南省教育厅科学研究基金项目(08C0234)

摘　　要：目的:研究木蝴蝶对酪氨酸酶的抑制及抗氧化作用。方法:以木蝴蝶醇提物的不同极性组分为研究对象,测定了木蝴蝶对酪氨酸酶的抑制作用,以及运用1,1-二苯-2-苦肼自由基(DPPH·)、羟基自由基(·OH)测定其抗氧化能力。结果:木蝴蝶醇提物的乙酸乙酯组分(EAO)、正丁醇组分(n-BO)、水提取组分(WO)对酪氨酸酶活性均有抑制作用,其半数抑制率(IC50)分别为3.081 4,8.525 3,22.750 8 g·L-1;DPPH·和·OH试验显示EAO,n-BO,WO具有较强的自由基清除作用,其清除自由基能力强弱次序均为EAO>n-BO>WO。结论:木蝴蝶具有良好的酪氨酸酶抑制活性及抗氧化作用,抑制酪氨酸酶活性的生成与其抗氧化能力具有正相关性;木蝴蝶醇提物的功能性成分主要分布在乙酸乙酯相中。Objective：To study anti-tyrosinase and antioxidation activities of extract from Oroxylum indicum.Method：This paper took different polar fractions of extract from the O.indicum ethanolic extract as research objects,measured its inhibition of tyrosinase and 1,1-diphenyl-2-picrylhydrazyl free radical （DPPH ·）and the hydroxyl free radical （· OH） scavenging ability.Result：Its alcohol extract of ethyl acetate extract （EAO）,n-butanol extract （n-BO） and water extract （WO） had inhibition to tyrosinaes,the values of IC50 reached 3.081 4,8.525 3,22.750 8 g ·L-1 respectively; EAO,n-BO,WO had stonger scavenging effect of DPPH ·and · OH,the order of scavenging ability was EAO ＞n-BO ＞WO.Conclusion：O.indicum.had better tyrosinase inhibitory and antioxidation,its antioxidation ability had positive correlation with its anti-tyrosinase.Its functional components were mainly distributed in the ethyl acetate phase.

关 键 词：木蝴蝶 酪氨酸酶 抑制 自由基 清除 抗氧化 

分 类 号：R284.1[医药卫生—中药学]