HPLC双波长法同时测定净固片中5种活性成分的含量  

Simultaneous Determination of 5 Active Ingredients in Jinggu Tablets by HPLC Dual-wavelength Detection

作　　者：曾琪[1,2] 谭道鹏 陈艳萍[1] 曾伟珍 严启新 曾令杰[1] 

机构地区：[1]广东药学院中药学院,广州510006 [2]深圳海王药业有限公司,广东深圳518057

出　　处：《中国实验方剂学杂志》2014年第18期77-80,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：2008年企业技术中心创新能力专项项目(发改办高技[2008]2012号)

摘　　要：目的：建立HPLC双波长法同时测定净固片中芦丁、柚皮苷、橙皮苷、新橙皮苷、槲皮素的方法.方法：依利特Kromasil-C18色谱柱（4.6 mm×250 mm,5μm）,流动相0.2％冰醋酸（A）-乙腈（B）梯度洗脱（0 ～ 10 min,12％ ～15％B;10～15 min,15％ ～17％B;15～25 min,17％B;25～35 min,17％ ～20％B;35～45 min,20％ ～22％ B;45～60 min,22％ ～40％B）,柱温30℃,进样量10 μL,流速1.0 mL· min-1,芦丁和槲皮素检测波长为254 nm,柚皮苷、橙皮苷和新橙皮苷检测波长为283 nm.结果：芦丁在0.012 5 ～0.500 4 g·L-1（r=1.000 0）,柚皮苷在0.010 0～0.400 8 g·L-1（r=0.999 9）,橙皮苷在0.003 3 ～0.032 7 g·L-1（r=0.999 2）,新橙皮苷在0.011 3 ～0.225 6 g·L-1（r =0.999 9）,槲皮素在0.0041 ～0.081 3 g·L-1（r=0.9998）与峰面积具有良好的线性关系,平均回收率均在98％ ～102％.结论：该方法准确可靠,可行性及重复性良好,可作为净固片的质量控制方法.Objective：The study was conducted to establish a HPLC method for simultaneously determination of rutin,naringin,hesperidin,neohesperidin and quercetin in Jinggu tablet.Method：The separation of compounds was performed on Elite Kromasil-C18 column （4.6 mm × 250 mm,5 μm）,with the mobile phase consisted of acetonitrile-0.2％ acetic acid.The flow rate was set at 1.0 mL ·min-1and detection wavelengths were set at 254 nm for rutin and quercetin,283 nm for naringin,hesperidin and neohesperidin.Result：The samples showed a good relationship in the ranges of 0.012 5-0.500 4 g ·L-1 for rutin,0.010 0-0.400 8 g ·L-1 for naringin,0.003 3-0.032 7 g ·L-1 for hesperidin,0.011 3-0.225 6 g ·L-1 for neohesperidin and 0.004 1-0.081 3g ·L-1 for quercetin.All of the average recoveries were between 98％ and 102％.Conclusion：The method is accurate,reliable and reproducible and can be used for the quality control of Jinggu tablet.

关 键 词：高效液相色谱 净固片 芦丁 柚皮苷 橙皮苷 新橙皮苷 槲皮素 

分 类 号：R284.1[医药卫生—中药学]