加味独活寄生汤对胶原诱导性关节炎大鼠关节炎症的影响  

Effect of Jiawei Duhuo Jisheng Decoction on Joint Arthritis of Type ⅡCollagen-induced Arthritis in Rats

作　　者：马卫国[1] 刘慧[1] 张春艳[1] 孟凤仙[1] 陆妍[1] 王亚南[1] 陈祎[2] 林子超[1] 高双荣[3] 王海林[3] 

机构地区：[1]北京中医药大学东方医院,北京100078 [2]北京中医药大学第三附属医院,北京100029 [3]中国中医科学院中药研究所,北京100700

出　　处：《中国实验方剂学杂志》2014年第18期155-159,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：北京中医药大学自主选题资助项目(2013-JYBZZ-JS-093)

摘　　要：目的:观察加味独活寄生汤对Ⅱ型胶原诱导性关节炎(CIA)大鼠关节炎症的影响。方法:SPF级雄性SD大鼠90只,随机分为正常对照组、模型对照组、来氟米特治疗组、加味独活寄生汤高、中、低剂量组共6组。除正常对照组6只,于大鼠尾根部每只0.2mg im胶原乳剂制成关节炎大鼠模型,造模后,每组选择造模成功的大鼠分别6只,来氟米特组按10 mL·kg-1给予来氟米特ig,加味独活寄生汤高、中、低剂量组按44,22,11 g·kg-1给予加味独活寄生汤ig,对照组ig相同体积的蒸馏水,每天1次,连续12周。分别观察治疗后0,2,4,6,8,10,12周大鼠体质量、关节炎症指数(AI)、足爪指标、关节病理变化情况。结果:与模型组比较,加味独活寄生汤高、中、低剂量组与来氟米特组对关节炎大鼠AI有显著改善作用,差异有统计学意义(P<0.05,P<0.01);各治疗组较模型组体质量、足爪均有显著改善(P<0.05,P<0.01);对踝关节软骨病理改善方面,高剂量组与模型组比较有改善。结论:加味独活寄生汤对CIA大鼠关节炎症有明显改善作用,是治疗类风湿关节炎的有效方剂。Objective：To study the effect of Jiawei Duhuo Jisheng decoction in collagen-induced arthritis （CIA） rats.Method：Ninety male SPF Sprague-Dawley rats were randomly divided into the model control group,the leflunomide group （ig,10 mL ·kg-1）,and the low-,medium-,high-dosage groups of Jiawei Duhuo Jisheng decoction （ig,11,22,44 g ·kg-1） of 6 rats each group.The CIA model was established except rats in the model control group by injection of 0.2 mg complete Freund＇s adjuvant at the end of the tail of rats.The rats in normal model group were given the same dosage of distilled water.All rats received oral administration of corresponding medicines once daily for 12 weeks.The body weight,arthritis index （AI）,paw index,joint pathological changes of rats were observed at 0,2,4,6,8,10 and 12 weeks after treatment.Result：The AI of rats in the low-,medium-,high-dosage Jiawei Duhuo Jisheng decoction groups and the leflunomide group have significant improvement,there was statistically significant difference as compared with that in the model control group （P ＜0.05,P ＜ 0.01）.The body weight,paw index changes were significantly improved after treatment than the model control group （P ＜ 0.05,P ＜ 0.01）.The ankle joint cartilage pathological had an improvement in the high-dosage Jiawei Duhuo Jisheng decoction groups as compared with the control model group.Conclusion：Jiawei Duhuo Jisheng decoction can significantly improve the joint arthritis of CIA rats,which is an effective prescription used for treating rheumatoid arthritis.

关 键 词：关节炎 类风湿 加味独活寄生汤 

分 类 号：R285.5[医药卫生—中药学]