茶树油眼用凝胶和甲硝唑滴眼液体外抗螨作用比较  

An Experiment Study of Killing Ocular Demodex in vitro with Tea Tree Oil Ocular Gel and Arilin Eye Drops

作　　者：韩雷[1,2] 皇甫赟 王应飞[1,2] 张俊杰[1,2] 王丽娅[1,2] 

机构地区：[1]河南省立眼科医院 [2]河南省眼科研究所,郑州450003 [3]河南医学高等专科学校,郑州451191

出　　处：《中国实验方剂学杂志》2014年第18期188-191,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：河南省科技厅研究开发专项基金项目(豫科攻072103810601);河南医学科技攻关项目(豫卫科200703092)

摘　　要：目的:探讨5%茶树油凝胶、2%甲硝唑滴眼液2种制剂对毛囊蠕形螨的体外杀灭作用,为选择有效的灭螨方法提供依据。方法:在本院眼科门诊用睫毛拔取法采集眼部毛囊蠕形螨,置于细胞板中,滴加5%茶树油凝胶、2%甲硝唑滴眼液,利用倒置显微镜观察其存活情况,生理盐水为空白对照。结果:5%的茶树油凝胶对毛囊蠕形螨有较好的杀灭作用,虫体形态及活动度均发生了兴奋-痉挛-松弛-死亡的典型变化。毛囊蠕形螨在5%茶树油凝胶中的存活时间平均为(1.43±0.62)h,与2%甲硝唑滴眼液组的平均存活时间(27.65±5.14)h比较差异有统计学意义(P<0.01)。毛囊蠕形螨在3组间的存活时间经H检验,差异有统计学意义(P<0.01)。其中,毛囊蠕形螨在2%甲硝唑滴眼液中的存活时间与空白对照组的平均存活时间(23.33±3.98)h比较差异无统计学意义。毛囊蠕形螨在5%茶树油凝胶与空白对照组的存活时间比较差异有统计学意义(P<0.01)。在5%茶树油凝胶、2%甲硝唑滴眼液和空白对照组间,毛囊蠕形螨24 h死亡率分别为100%,20%,50%,差异具有统计学意义(P<0.01)。结论:2%甲硝唑滴眼液对毛囊蠕形螨无体外杀灭作用;5%茶树油凝胶对毛囊蠕形螨有体外杀灭作用。茶树油凝胶制剂可作为一种天然高效的杀螨药物。Objective：The purpose of this study was to compare the acaricidal effect of 5％ tea tree oil gel and 2％ arilin eye drops on the ocular Demodexfolliculorum.Method：Eyelash samples were epilated from outpatient of ophthalmology to collect ocular D.folliculorum,transferred into 6-well plates and examined under the inverted microscope.Each well contained at least 20 live mites which were collected and treated with 5％ tea tree oil gel and 2％ arilin eye drops,respectively,with normal saline as blank control.Result：5％ tea tree oil gel was demonstrated to show better killing effect on the mite in vitro.After drug treatment,the morphology and activity of D.folliculorum altered with typical changes of excitation-spasm-chalasia-death.In the treatment of 5％ tea tree oil gel,the average survival time of mites was （1.43 ±0.62） h,while in 2％ arilin eye treatment,it was （27.65 ±5.14） h,with a significant difference between them （P ＜ 0.01）.The survial times of mites,showed significant difference among the three groups （P ＜ 0.01）.The survival time of D.folliculorum in normal saline group was （23.33 ±3.98） h,which had significant difference from the 5％ tea tree oil gel group （P ＜0.01） ; while in the 2％ arilin eye drops group the survival time was （27.65 ± 5.14） h,compared with the survival time of control group,there was no significant difference between them.The mortality of mites within 24 hours in 5％ tea tree oil gel,2％ arilin eye drops and control group were 100％,20％ and 50％ respectively,and there was a significant difference between them （P ＜0.01）.Conclusion：2％ arilin eye drops has no effect on killing D.folliculorum in vitro,while 5％ tea tree oil gel has the killing effect on D.folliculorum in vitro.Tea tree oil gel could be developed as a natural drug to kill ocular D.folliculorum in high-efficency.

关 键 词：茶树油凝胶 甲硝唑滴眼液 毛囊蠕形螨 存活时间 24 h死亡率 

分 类 号：R287.6[医药卫生—中药学]