高压氧治疗对神经病理性痛大鼠脊髓神经生长因子表达的影响  

Effects of hyperbaric oxygen treatment on expression of nerve growth factor in spinal cord of rats with neuropathic pain

作　　者：赵柏松 孟凌新[2] 谭永红 宋兴荣 

机构地区：[1]广州市妇女儿童医疗中心麻醉科,广州市510623 [2]中国医科大学附属盛京医院麻醉科

出　　处：《中华麻醉学杂志》2014年第7期800-803,共4页Chinese Journal of Anesthesiology

基　　金：贝朗麻醉科学研究基金（BBF2012-012）

摘　　要：目的 评价高压氧治疗对神经病理性痛大鼠脊髓神经生长因子（NGF）表达的影响.方法 成年雄性SD大鼠30只,体重270 ～ 300 g,8～ 10周龄,采用随机数字表法,将其分为5组（n=6）：假手术组（S组）、神经病理性痛组（NP组）、纯氧组（O组）、2.5标准大气压高压氧治疗组（H2.5组）和3.0标准大气压高压氧治疗组（H3.0组）.采用坐骨神经慢性压迫性损伤法建立神经病理性痛模型,H2.5组和H3.0组于术后1d开始给予相应压力的高压氧治疗,1次/d,连续7d.于术后3、5、7和14 d出高压氧舱后1h时测定机械缩足反应阈（MWT）和热缩足反应潜伏期（TWL）.于术后14 d测定痛阈后处死,采用免疫组化法和Western blot法测定脊髓NGF表达水平.结果 与S组比较,NP组和O组术后各时点MWT降低,TWL缩短,脊髓NGF表达上调（P＜0.05）.与NP组比较,H2.5组和H3.0组术后各时点MWT升高,TWL延长,脊髓NGF表达上调（P＜0.05）,O组MWT、TWL和脊髓NGF表达差异无统计学意义（P＞0.05）.与H2.5组比较,H3.0组脊髓NGF表达下调（P＜0.05）,MWT和TWL差异无统计学意义（P＞ 0.05）.结论 高压氧治疗减轻大鼠神经病理性痛的机制与上调脊髓NGF表达有关.Objective To evaluate the effect of hyperbaric oxygen （HBO） treatment on the expression of nerve growth factor （NGF） in the spinal cord of rats with neuropathic pain （NP）.Methods Thirty healthy male Sprague-Dawley rats,aged 8-10 weeks,weighing 270-300 g,were randomly divided into 5 groups （n =6 each） using a random number table：sham operation group （group S）,group NP,pure oxygen group （group O）,treatment with HBO at 2.5 atmosphere absolute group （group H2.5） and treatment with HBO at 3.0 atmosphere absolute group （group H3.0）.The animals were anesthetized with intraperitoneal 10% chloral hydrate 300 mg/kg.NP was induced by chronic constrictive injury.The left sciatic nerve was exposed and 4 loose ligatures were placed on the sciatic nerve at 1-mm intervals with 4-0 silk thread.HBO treatment was performed with the corresponding atmosphere absolute once a day for 7 consecutive days starting from 1 day after operation in H2.5 and H3.0 groups.The mechanical paw withdrawal threshold （MWT） and thermal paw withdrawal latency （TWL） were measured on 3,5,7 and 14 days after operation.The animals were sacrificed after the last measurement of pain threshold,and the lumbar segments of the spinal cord were removed to detect the expression of NGF by immuno-histochemistry and Western blot analysis.Results Compared with group S,MWT was significantly decreased,TWL was shortened,and the expression of NGF was up-regulated at each time point after operation in NP and O groups.Compared with group NP,MWT was significantly increased,TWL was prolonged,and the expression of NGF was up-regulated at each time point after operation in H2.5 and H3.0 groups,and no significant change was found in MWT,TWL and expression of NGF in group O.Compared with group H2.5,the expression of NGF was significantly down-regulated,and no significant change was found in MWT and TWL in group H3.0.Conclusion The mechanism by which HBO treatment mitigates NP is related to up-regulation of the expression of NGF in the spina

关 键 词：高压氧 神经痛 神经生长因子 脊髓 

分 类 号：R473.72[医药卫生—护理学]