T-2毒素对小鼠睾丸间质细胞相关功能基因mRNA表达的影响  

Effects of T-2 toxin on mRNAs expression of related functional genes in primarily cultured mouse Leydig cells

作　　者：杨建英[1] 张勇法[2] 白雪飞[1] 符志鹏[1] 辛淑文 闫彬[1] 

机构地区：[1]河南科技大学医学技术与工程学院医学检验系,河南洛阳471003 [2]河南科技大学食品与生物工程学院生物工程系

出　　处：《环境与健康杂志》2014年第6期477-480,共4页Journal of Environment and Health

基　　金：国家自然科学基金(31201119);河南省高校青年骨干教师项目(2013GGJS-067);河南省科技厅自然科学研究计划项目(112102310144);河南省教育厅自然科学研究计划项目(2011B310004)

摘　　要：目的探讨T-2毒素对小鼠睾丸间质细胞相关功能基因mRNA表达的影响。方法对分离出的4~5周龄BABL/c小鼠睾丸间质细胞进行体外培养,分别暴露于含10 ng/ml hCG（对照）和10-9、10-8、10-7mol/L T-2毒素＋10 ng/ml hCG的培养液中24h。采用RT-PCR方法检测相关功能基因（3β-HSD-1,P450scc和StAR）mRNA的表达水平。结果与对照组相比,10 ng/ml hCG＋10-8mol/L T-2毒素和10 ng/ml hCG＋10-7mol/L T-2毒素染毒组小鼠睾丸间质细胞3β-HSD-1 mRNA的表达水平及10 ng/ml hCG＋各剂量T-2毒素染毒组小鼠睾丸间质细胞P450scc/HPRT、StAR/HPRT mRNA的表达水平均下降,差异有统计学意义（P〈0.05,P〈0.01）;且随着T-2毒素染毒剂量的升高,10 ng/ml hCG诱导小鼠睾丸间质细胞3β-HSD-1、P450scc和StAR mRNA的表达水平呈下降趋势。结论 T-2毒素可通过抑制小鼠睾丸间质细胞3β-HSD-1、P450scc和StAR mRNA表达而对雄性小鼠产生生殖毒性。Objective To study the effects of T-2 toxin on the mRNAs expression of related functional genes in primarily cultured mouse Leydig cells. Methods Leydig cells i, olated from BABL/c mice aged 4-5 weeks were cultured primarily and treated with T-2 toxin at the doses of 10 ng/ml hCG, 10-9 mol/L T-2 toxin ＋10 ng/ml hCG, 10-8 mol/L T-2 toxin ＋10 ng/ml hCG, 10-7 mol/L T-2 toxin＋10 ng/ml hCG for 24 h. The mRNA expression levels of related functional genes in the groups were detected after 24 h incubation by quantitative polymerase chain reaction （RT-PCR）. Results The expression levels of P450scc and StAR mRNA in Leydig cells in 10-9,10-8, 10-7 mo[/L T-2 toxin＋10 ng/ml hCG groups, expression levels of 3β-HSD-1 in 10-8, 10-7 mol/L T-2 toxin＋10 ng/ml hCG groups,were significantly lower than those in the control group （P〈0.05）. With the increases of T-2 toxin exposure doses, the expresson levels of 313-HSD-1,P450scc and StAR mRNA in Leydig cells decreased. Conclusion T-2 toxin exposure may inhibit the mRNA expression of 3β-HSD-1 ,P450scc and StAR in Leydig cells in mice ,which indicates that T-2 toxin has reproductive toxicity to male mice.

关 键 词：T-2毒素 生殖毒性 间质细胞 

分 类 号：R994.6[医药卫生—毒理学]