分散紫HFRL和分散橙S-4RL对大型溞的单一与联合急性毒性效应  

Single and joint acute toxicity of disperse violet HFRL and disperse orange S-4RL to Daphnia magna

作　　者：王焕双 李玲[1] 吴贵江[1] 魏燕芳[1] 

机构地区：[1]华侨大学化工学院,福建厦门361021

出　　处：《环境与健康杂志》2014年第6期483-485,共3页Journal of Environment and Health

基　　金：华侨大学高层次人才科研启动项目(12BS108);福建省自然科学基金计划资助项目(2014J01049)

摘　　要：目的以大型溞（Daphniamagna）为试验生物,研究分散紫HFRL和分散橙S-4RL对大型溞的单独急性毒性及联合急性毒性。方法采用大型溞毒性试验标准方法,设置5个浓度梯度及空白对照,测定分散紫HFRL（12.5-200 mg/ml）和分散橙S-4RL（0.012 5-0.2 mg/ml）及其混合物（0.000 8-0.5 mg/ml分散紫HFRL和0.000 5-0.8 mg/ml分散橙S-4RL）对大型溞的24h及48 h急性毒性。采用毒性单位法（TU）、相加指数法（AI）、混合毒性指数法（MTI）以及毒性增大指数（TEI）评价联合毒性。结果分散紫HFRL的48 h-LC50为177.93 mg/L;分散橙S-4RL的24 h及48 h-LC50分别为0.05和0.03mg/L。分散橙S-4RL和分散紫HFRL配比为1∶4、2∶3、1∶1、3∶2、4∶1时的24 h-LC50mix分别为0.05、0.11、0.09、0.12、0.28mg/L;48 h-LC50mix分别为0.02、0.03、0.02、0.05、0.07mg/L。不同联合毒性评价方法结果相同：当毒性单位配比为1∶4及2∶3时,表现为弱协同作用,而配比为1∶1、3∶2以及4∶1时表现为拮抗作用。结论本次研究的两种染料的不同配比对大型溞的毒性不同,一般呈现拮抗作用;三种联合毒性评价方法结果一致。Objective To understand the single and joint toxicity of disperse orange S-4RL and disperse violet HFRL to Daphnia magna. Methods Daphnia magna was exposed to the disperse orange S-4RL（0.012 5-0.2 mg/ml）,disperse violet HFRL （12.5-200 mg/ml） and their mixtures at five levels （0.000 8-0.5 mg/ml disperse violet HFRL, 0.000 5-0.8 mg/ml disperse orange S-4RL）, and one control group was set. 24 h and 48 h-LCs0 were measured. Toxicity unit （TU）, additional index （AI）, mixture toxicity index （MTI） and toxicity enhancement ildex （TEI） were used to evaluate the joint toxicities. Results Disperse violet HFRL 48 h-LC50 for Daphnia magna was 177.93 rag/L, disperse orange S-4RL 24 h-LC50 and 48 h-LC50 were 0.05 mg/L and 0.03 mg/L respectively. For mixtures, 24 h-LC0mix were 0.05,0.11,0.09,0.12 and 0.28 mg/L, 48 h-LC50mix were 0.02,0.03,0.02,0.05 and 0.07 nag/L, at ratio （disperse orange S-4RL to disperse violet HFRL） of 1：4,2：3,1：1,3：2 and 4：1 respectively. Based on TU ,AI ,MTI,the joint toxicity showed weak cooperativity at ratios （disperse orange S-4RL to disperse violet HFRL） of 1：4 and 2：3 ,and antagonism at ratios of 1：1,3：2 and 4：1. Conclusion Mixtures with different ratios have different toxic effects which are mainly antagonism.The j~}int toxicities of mixtures show the same based on TU ,AI and MTI.

关 键 词：染料 分散紫HFRL 分散橙S-4RL 大型溞 联合毒性 

分 类 号：R994.6[医药卫生—毒理学]