短期锰暴露对成年雄性大鼠睾丸内氨基酸类神经递质的影响  

Effects of short-term exposure to manganese on amino acid neurotransmitters in testis of adult rats

作　　者：施文祥[1] 李少军[2] 李胜联[1] 欧超燕[1] 

机构地区：[1]桂林医学院公共卫生学院,广西桂林541004 [2]广西医科大学公共卫生学院

出　　处：《环境与健康杂志》2014年第6期493-495,共3页Journal of Environment and Health

基　　金：桂林医学院高层次人才科研启动基金(KY2012092)

摘　　要：目的探讨短期锰暴露对大鼠睾丸内谷氨酸(Glu)、γ-氨基丁酸(GABA)、谷氨酰胺(Gln)含量的影响。方法将32只健康成年SPF级雄性SD大鼠随机分为4组,分别为对照(无菌生理盐水)组和7.5、15、30 mg/kg氯化锰染毒组,每组8只。采用腹腔注射方式进行染毒,每天1次,每周5次,连续染毒4周。测定大鼠睾丸组织内Glu、GABA、Gln含量。结果与对照组比较,30 mg/kg氯化锰染毒组大鼠睾丸内Gln、GABA含量及15、30 mg/kg氯化锰染毒组大鼠睾丸内Glu含量均较低,差异有统计学意义(P<0.05)。随着氯化锰染毒浓度的升高,大鼠睾丸内Glu、Gln、GABA的含量均呈先升高后下降的趋势。结论过量锰暴露可影响雄性小鼠性腺的GABA能系统,导致睾丸组织的Glu、Gln及GABA的含量降低。Objective To understand the effects of short-term exposure to manganese on amino acid neurotransmitters, including glutamate （Glu）,glutamine （Gln）,gamma-am nobutyric acid （GABA） in the testis of male rats. Methods Thirty-two SD male rats were randomly divided into the control,7.5,15,30 mg/kg body weight MnC12 groups. The rats in experiment groups received daily intraperitoneally injections of MnCl2 solution,while the rats in control group received daily intraperitoneally injections of normal saline （NS）,once a day,five days per week,for four consecutive weeks. The concentrations of Glu, Gin and GABA in testis of rat were detected b,, the high performance liquid chromatography. Results After four-week exposure,compared with the control,the concentrations＇ of Gin and GABA in testis in 30 mg/kg MnClz-exposure group and the concentrations of Glu in testis of 15,30 mg/kg MnC12-exposure groups decreased （P〈0.05）. Conclusion Excessive manganese exposure may affect the concentration of Glu, Gln and GABA in testis of adult rats.

关 键 词：锰 睾丸 Γ-氨基丁酸 谷氨酸 谷氨酰胺 

分 类 号：R994.6[医药卫生—毒理学]